生长方案:	-
处理方案:	Incubated with CD3 antibody; Incubated with Isotype control; Treated with Ustekinumab incubated with CD3 antibody; Treated with Ustekinumab incubated with Isotype control; Untreated with Ustekinumab incubated with CD3 antibody; Untreated with Ustekinumab incubated with Isotype control
提取方案:	Skin is sampled, treated with antibodies. The days after epidermis and dermis are separated with dispase, then flash frozen on dry ice. RNA is extracted with Qiazol (qiagen) and send for sequencing. After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA(for eukaryotes) or by removing rRNAs from the total RNA (for prokaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. After agarose gel electrophoresis, the suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using Illumina HiSeqTM 2000 or other sequencer when necessary.
建库方案:	RNA libraries were prepared for sequencing using standard Illumina protocols

分子类型:	poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2000
链特异性:	Unspecific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度