PRJNA415913: Transcriptomic data of MDA-MB-231 cells adapted to culture in media containing different sugars (glucose or fructose) and cultured as mammospheres
概要: We report the single-cell RNA sequencing data obtained from MDA-MB-231 breast cancer cells cultured in standard DMEM with 25 mM glucose, or adapted to culture in DMEM with 10 mM fructose to reduce glycolysis, and then cultured as mammospheres
项目整体设计: Examination of transcriptomic changes in MDA-MB-231 breast cancer cells mammospheres in response to restriction of glycolysis
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MDA-MB-231 cells were adapted to culture in 2D in DMEM containing either 25 mM glucose or 10 mM fructose, for 30 days. |
| 处理方案: |
Adapted cell cultures were plated on low attachment plates in conditioned media from their respective 2D cultures. Resultant mammospheres were cultured for 10 days |
| 提取方案: |
Trypsin/ EDTA disaggregated mammospheres.PCR on bead then Nextera XT |
| 建库方案: |
- |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
Forward |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina NextSeq 550 |
| 链特异性: |
Specific |
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加标(Spike-In) |
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The effects of restricted glycolysis on stem-cell like characteristics of breast cancer cells.
Oncotarget . 2018-05-01 [PMID:
29796188]