生长方案:	Primary normal human bronchial epithelial (NHBE) cells were purchased from Lonza and cultured in bronchial epithelial growth medium (BEGM) (Lonza) to passage 3. The cells were harvested and seeded on decellularized bronchial scaffolds where they were allowed to attach and grow for 4 days in BEGM. Differentiation was induced after 4 days by exchanging the BEGM with a differentiation medium (50 % BEGM, 50% Dulbecco's Modified Eagle's Medium and 0.05 µM retinoic acid) and differentiation proceeded during 35 days.
处理方案:	Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 healthy donors. Differentiation was induced 4 days later and repopulated scaffolds were collected after 0 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 healthy donors. Differentiation was induced 4 days later and repopulated scaffolds were collected after 7 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 healthy donors. Differentiation was induced 4 days later and repopulated scaffolds were collected after 14 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 healthy donors. Differentiation was induced 4 days later and repopulated scaffolds were collected after 21 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 healthy donors. Differentiation was induced 4 days later and repopulated scaffolds were collected after 28 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 healthy donors. Differentiation was induced 4 days later and repopulated scaffolds were collected after 35 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 COPD patients. Differentiation was induced 4 days later and repopulated scaffolds were collected after 0 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 COPD patients. Differentiation was induced 4 days later and repopulated scaffolds were collected after 7 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 COPD patients. Differentiation was induced 4 days later and repopulated scaffolds were collected after 14 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 COPD patients. Differentiation was induced 4 days later and repopulated scaffolds were collected after 21 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 COPD patients. Differentiation was induced 4 days later and repopulated scaffolds were collected after 28 days of differentiation.; Normal bronchial epithelial (NHBE) cells were seeded on decellularized bronchial scaffolds from 3 COPD patients. Differentiation was induced 4 days later and repopulated scaffolds were collected after 35 days of differentiation.
提取方案:	Total RNA was extracted using the RNeasy Mini kit (Qiagen).
建库方案:	560 ng total RNA/sample was used as input to create cDNA libraries using the TruSeq Stranded mRNA kit (Illumina) with dual indexing following standard instructions.

分子类型:	poly(A)+ RNA
库的片段类型:	SINGLE
库的链类型:	Forward
测序平台:	ILLUMINA
测序仪型号:	Illumina NextSeq 500
链特异性:	Specific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度