概要: We generated expression profiles of TH1 and TREG cells from T1D and healthy subjects by RNA-Seq. By integrating RNA-Seq dta with other data sets, we predicted and validated serveral T1D risk SNPs.
项目整体设计: Effector memory TH1 and effector memory TREG cells were isolated from 6 T1D patients and mached 5 helathy controls. Gene expression profilings were generated using RNA-Seq. By integrating RNA-Seq data, ChIP-Seq and other data sets, we predicted and validated several risk SNPs for T1D.
TH1 and TREG cell were sorted into TRIzol-LS and total mRNA was isolated using RNeasy Micro Kit (Qiagen). Total RNA was treated with DNase (Ambion) to remove genomic DNA.
建库方案:
Libraries were prpared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) according to manufacturer's instructions. Bioanalyzer, KAPA qPCR were done before pooling libraries for sequencing on Illumina HiSeq2500 in paired-end mode with the read length of 75 nt.
测序信息
分子类型:
poly(A)+ RNA
库的片段类型:
PAIRED
库的链类型:
-
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 2500
链特异性:
Unspecific
样本
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生物条件:
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质量评估:
数据来源
GEN样本编号
GEN数据集编号
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物种
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组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
表型
Condition Detail
生长方案
处理方案
提取方案
建库方案
分子类型
库的片段类型
链特异性
库的链类型
加标（Spike-In）
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细胞数
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平均测序片段长度_1
平均测序片段长度_2
唯一比对率
多重比对率
覆盖度
文章
Risk variants disrupting enhancers of TH1 and TREG cells in type 1 diabetes.
Proceedings of the National Academy of Sciences of the United States of America . 2019-03-25 [PMID:
30910956]
