概要: Purpose: To assess global changes of wheat coleoptiles gene expression in response to fusaoctaxin A which is a linear, C-terminally-reduced and D-amino acid residue-rich octapeptide, as the product of the two non-ribosomal peptide synthetases encoded by Fusarium graminearum fg3_54 cluster.Methods: For RNA seq of wheat coleoptiles, three biological replicates of mock-inoculated coleoptiles, wild-type PH-1 inoculated coleoptiles (3 dpi), ∆fg3_54 inoculated coleoptiles (3 dpi), after 3 hours fusaoctaxin A-treated coleoptiles, after 3 hours control (0.5% Tween-20) treated coleoptiles, after 24 hours fusaoctaxin A-treated coleoptiles and after 24 hours control (0.5% Tween-20) treated coleoptiles were sequenced.Results: For each sample, 33-95 M RNA-seq clean reads were obtained mapped to the Triticum aestivum Chinesespring genome sequence.Our results of RNA sequencing indicated fusaoctaxin does not induce PR gene expression, but suppress plant immunity and chloroplastc activity.Conclusions: Fusaoctaxin A represents an unprecedented virulence factor which can manipulate chloroplast and suppress host immunity.
项目整体设计: Three biological replicates of mock-inoculated coleoptiles, wild-type PH-1 inoculated coleoptiles (3 dpi), ∆fg3_54 inoculated coleoptiles (3 dpi), after 3 hours fusaoctaxin A-treated coleoptiles, after 3 hours control (0.5% Tween-20) treated coleoptiles, after 24 hours fusaoctaxin A-treated coleoptiles and after 24 hours control (0.5% Tween-20) treated coleoptiles were sequenced using Illumina NovaSeq 6000 Sequencing System.
Grow the wheat seedling in the growth chamber for 3 day under controlled environment conditions at 25 °C with a 12 h light/12 h dark photoperiod
处理方案:
For Fusarium graminearum inoculation: Mock, wild-type PH-1 and △fg3_54 inoculate wheat seedling. For compound treatment: add 2μl fusaoctaxin A (0.5mM) or 0.5% tween-20 onto wound site of wheat coleoptiles.
提取方案:
Total RNA was isolated using EasyPure Plant RNA kit (Transgen biotech) according to the manufacturer’s protocol. RNA integrity was confirmed using the 2100 Bioanalyzer (Agilent Technologies) with a minimum RNA integrated number (RIN) value of 7.
建库方案:
RNA libraries were prepared for sequencing using standard Illumina protocols
测序信息
分子类型:
poly(A)+ RNA
库的片段类型:
PAIRED
库的链类型:
-; Forward
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 2500; Illumina NovaSeq 6000
链特异性:
Unspecific; Specific
样本
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数据来源
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疾病
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突变/变异
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Condition Detail
生长方案
处理方案
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分子类型
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加标（Spike-In）
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平均测序片段长度_1
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文章
A linear nonribosomal octapeptide from Fusarium graminearum facilitates cell-to-cell invasion of wheat.
