PRJNA487796: Integrated Multi-omic Analysis of Esthesioneuroblastomas Identifies Two Subgroups Linked to Cell Ontogeny
概要: We report here the multi-omics analysis of esthesioneuroblastomas, which is a rare cancer arising from the olfactory bulb. We discovered ENB can be grouped in two subtypes, namely basal and neural ENBs. We also uncovered high rates of mutations (~35%) in basal ENB subgroup.
项目整体设计: We report here the multi-omics analysis of esthesioneuroblastomas using whole-exome sequencing (n=27), RNA sequencing (n=19) and DNA methylation profiling (n=26).
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- |
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RNA extraction was performed using the RNeasy Kit (Qiagen) according to the manufacturer¡¯s instructions. |
| 建库方案: |
Libraries were quantitated by qPCR (KAPA Systems), multiplexed and sequenced, 4 samples per lane, on the HiSeq2000 using 75 bp paired-end sequencing. |
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rRNA- RNA |
| 库的片段类型: |
PAIRED |
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| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina HiSeq 2000 |
| 链特异性: |
Unspecific |
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种族 |
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细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
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发育阶段 |
突变/变异 |
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Condition Detail |
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加标（Spike-In） |
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碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
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Integrated Multi-omic Analysis of Esthesioneuroblastomas Identifies Two Subgroups Linked to Cell Ontogeny.
Cell reports . 2018-10-01 [PMID:
30332658]
