Gene Expression
Nebulas
Gene Expression NebulasSummary: We report here the multi-omics analysis of esthesioneuroblastomas, which is a rare cancer arising from the olfactory bulb. We discovered ENB can be grouped in two subtypes, namely basal and neural ENBs. We also uncovered high rates of mutations (~35%) in basal ENB subgroup.
Overall Design: We report here the multi-omics analysis of esthesioneuroblastomas using whole-exome sequencing (n=27), RNA sequencing (n=19) and DNA methylation profiling (n=26).
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| Growth Protocol: | - |
| Treatment Protocol: | - |
| Extract Protocol: | RNA extraction was performed using the RNeasy Kit (Qiagen) according to the manufacturer¡¯s instructions. |
| Library Construction Protocol: | Libraries were quantitated by qPCR (KAPA Systems), multiplexed and sequenced, 4 samples per lane, on the HiSeq2000 using 75 bp paired-end sequencing. |
| Molecule Type: | rRNA- RNA |
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| Library Layout: | PAIRED |
| Library Strand: | - |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq 2000 |
| Strand-Specific: | Unspecific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Case Detail | Control Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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