PRJNA496578: Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Human 10x]
概要: IL-10 is a prototypical anti-inflammatory cytokine, which is fundamental to the maintenance of immune homeostasis, especially in the intestine. There is an assumption that cells producing IL-10 have an immunoregulatory function. However, here we report that IL-10-producing CD4+ T cells are phenotypically and functionally heterogeneous. By combining single cell transcriptome and functional analyses, we identified a subpopulation of IL-10-producing Foxp3Neg CD4+ T cells that displays regulatory activity unlike other IL-10-producing CD4+ T cells, which are unexpectedly pro-inflammatory. The combinatorial expression of co-inhibitory receptors is sufficient to discriminate IL-10-producing CD4+ T cells with regulatory function from others and to identify them across different tissues and disease models in mice and humans. These regulatory IL-10-producing Foxp3Neg CD4+ T cells have a unique transcriptional program, which goes beyond the regulation of IL-10 expression. Finally, we found that patients with Inflammatory Bowel Disease (IBD), demonstrate a deficiency in this specific regulatory T-cell subpopulation.
项目整体设计: We performed scRNA-seq analysis of IL-10-producing CD4+ T cells isolated from the blood (PBMCs) of two healthy donors
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Libraries were generated using the 10x Genomics Chromium platform |
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poly(A)+ RNA |
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PAIRED |
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Forward |
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ILLUMINA |
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Illumina HiSeq 4000 |
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Specific |
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Molecular and functional heterogeneity of IL-10-producing CD4+ T cells.
Nature communications . 2018-12-21 [PMID:
30575716]
