Gene Expression Nebulas
A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

Gene Expression Nebulas

A data portal of transcriptome profiles across multiple species

PRJNA496614: Genome-wide screening of drought-responsive long noncoding RNAs in rice

Source: NCBI / GSE121303
Submission Date: Oct 16, 2018
Release Date: Oct 16, 2019
Update Date: Oct 16, 2019

Summary: RNA-seq analyses of drought-treated rice leaves were performed to screen long noncoding RNAs showing differential expression in a time-dependent manner.

Overall Design: Eight transcriptome profiles of drought-treated 5-week-old rice leaves were generated by deep sequencing using Illumina HiSeq 4000.

GEN Datasets:
GEND000532
Strategy:
Species:
Tissue:
Healthy Condition:
Cell Type:
Cell Line:
Development Stage:
Protocol
Growth Protocol: Rice seeds were germinated on MS media at 28℃ for 4 days, and transplanted into soil pots (4x4x6 cm; 3 plants per pot) and grown in a greenhouse.
Treatment Protocol: Watering was withheld at 5 weeks after transplanting to soil and the soil water content in each pot was adjusted to approximately 75%. Soil moisture was monitored during the drought treatment using a Soil Moisture Sensor SM150.
Extract Protocol: Total RNAs were extracted from rice leaves using Trizol reagent, purified with RNeasy Mini Kit and treated with DNase I.
Library Construction Protocol: Strand-specific total RNA- and mRNA-seq libraries were prepared by TruSeq Stranded Total RNA Sample Preparation Guide and TruSeq Stranded mRNA Sample Preparation Guide, respectively.
Sequencing
Molecule Type: rRNA- RNA; poly(A)+ RNA
Library Source:
Library Layout: PAIRED
Library Strand: Forward
Platform: ILLUMINA
Instrument Model: Illumina HiSeq 4000
Strand-Specific: Specific
Samples
Basic Information:
Sample Characteristic:
Biological Condition:
Experimental Variables:
Protocol:
Sequencing:
Assessing Quality:
Data Resource GEN Sample ID GEN Dataset ID Project ID BioProject ID Sample ID Sample Name BioSample ID Sample Accession Experiment Accession Release Date Submission Date Update Date Species Race Ethnicity Age Age Unit Gender Source Name Tissue Cell Type Cell Subtype Cell Line Disease Disease State Development Stage Mutation Phenotype Condition Detail Growth Protocol Treatment Protocol Extract Protocol Library Construction Protocol Molecule Type Library Layout Strand-Specific Library Strand Spike-In Strategy Platform Instrument Model Cell Number Reads Number Gbases AvgSpotLen1 AvgSpotLen2 Uniq Mapping Rate Multiple Mapping Rate Coverage Rate