Gene Expression NebulasSummary: RNA-seq analyses of drought-treated rice leaves were performed to screen long noncoding RNAs showing differential expression in a time-dependent manner.
Overall Design: Eight transcriptome profiles of drought-treated 5-week-old rice leaves were generated by deep sequencing using Illumina HiSeq 4000.
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| Growth Protocol: | Rice seeds were germinated on MS media at 28℃ for 4 days, and transplanted into soil pots (4x4x6 cm; 3 plants per pot) and grown in a greenhouse. |
| Treatment Protocol: | Watering was withheld at 5 weeks after transplanting to soil and the soil water content in each pot was adjusted to approximately 75%. Soil moisture was monitored during the drought treatment using a Soil Moisture Sensor SM150. |
| Extract Protocol: | Total RNAs were extracted from rice leaves using Trizol reagent, purified with RNeasy Mini Kit and treated with DNase I. |
| Library Construction Protocol: | Strand-specific total RNA- and mRNA-seq libraries were prepared by TruSeq Stranded Total RNA Sample Preparation Guide and TruSeq Stranded mRNA Sample Preparation Guide, respectively. |
| Molecule Type: | rRNA- RNA; poly(A)+ RNA |
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| Library Layout: | PAIRED |
| Library Strand: | Forward |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq 4000 |
| Strand-Specific: | Specific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Condition Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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