Gene Expression
Nebulas
Gene Expression NebulasSummary: Cerebrospinal fluid transcriptional profiles in children with tuberculous meningitis (TBM) or other infections were compared using RNA-Seq and a biomarker signature driven by NMDA-receptor activation was identified
Overall Design: A total of 31 CSF samples were sequenced, which include 24 TBM cases (ventricular and lumbar puncture CSF) and 7 other infections (ventricular CSF only)
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| Growth Protocol: | - |
| Treatment Protocol: | All patients received standard antimycobacterial and adjunctive corticosteroid treatment |
| Extract Protocol: | Total RNA was extracted from patient whole blood collected in PAXgene tubes |
| Library Construction Protocol: | RNA-Seq libraries were constructed using NuGen Ovation Human FFPE Library Kit |
| Molecule Type: | rRNA- RNA |
| Library Source: | |
| Library Layout: | PAIRED |
| Library Strand: | Reverse; - |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq 4000 |
| Strand-Specific: | Specific; Unspecific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Condition Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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