PRJNA507252: Single-cell RNA-sequencing analysis of Arabidopsis roots
概要: We report the use of high-throughput single-cell RNA sequencing (scRNA-seq) to analyze gene expression in wild-type and mutant Arabidopsis root cells. We demonstrate that using a commercially available platform for droplet-based scRNA-seq (10X Genomics Chromium) enables transcriptional profiling of individual protoplasts representing all of the major cell/tissue types of the root. Furthermore, rare cell types and subtypes have been identified. These single-cell transcriptomes were also used to generate a pseudotime series for the root hair and non-hair cell differentiation pathways. In addition, scRNA-seq was used to define and compare transcriptomes from root epidermis mutants, which enabled a detailed molecular analysis of the mutant phenotype. This study demonstrates the feasibility and usefulness of scRNA-seq in plants and provides a gene expression map at single-cell resolution for the Arabidopsis root.
项目整体设计: Analysis of gene expression among WT(Col-0), hairless mutant(rhd6-1) and hairy mutant(gl2) at the single cell level
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Plants grow on MS media for 5 DAG (day after germination) |
| 处理方案: |
Wild type; Hairless mutant; Hairy mutant |
| 提取方案: |
After Isolating protoplasts from roots, load into 10X Genomics unit for single-cell cDNA library construction. |
| 建库方案: |
Libraries were prepared for sequencing using 10X Genomics protocols; R1 is technical and R2 contains the real biological information. |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
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| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina HiSeq 4000 |
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- |
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加标（Spike-In） |
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Single-Cell RNA Sequencing Resolves Molecular Relationships Among Individual Plant Cells.
Plant physiology . 2019-02-04 [PMID:
30718350]
