Growth Protocol:	-
Treatment Protocol:	chemotherapy: No; chemotherapy: Yes
Extract Protocol:	Non-involved lung, tumour tissues, and metastatic lesions were obtained from patients with lung adenocarcinoma undergoing resection surgery at Memorial Sloan Kettering Cancer Center (MSKCC, New York, NY) after obtaining informed consent. Tissue samples were immediately placed in RPMI media (Corning) or Hypothermosol on ice and dissociated using both mechanical and enzymatic digestion (Human Tumour Dissociation Kit #130-095-929, Miltenyi Biotec), generally within 1 h of surgical resection. Tissues were minced with a razor blade in the Miltenyi enzyme mix according to the manufacturer's specifications and transferred to a Gentle MACS Octo Dissociator with heaters (# 30-096-427, 37℃) for further mechanical dissociation. Upon completion, cell suspensions were passed through a 70 μm filter and washed twice with FACS buffer (2% heat-inactivated FBS, 1 mM EDTA and Pen/Strep in PBS without Ca or Mg). Red blood cells were lysed in Red Blood Cell Lysis Solution (ACK lysis buffer) once or twice depending on red blood cell content, and final single-cell suspensions were made in Hanks’ Balanced Salt Solution (HBSS). For scRNA-seq, the remaining cell suspensions were subsequently flow sorted with a BD FACSAria II cell sorter fitted with a 100 μM nozzle to enrich for viable, single cells according to forward and side scattering, and DAPI exclusion. Cells were sorted directly into RPMI media with 10% FBS, washed thrice and re-suspend in PBS with 0.04% BSA for single cell encapsulation.
Library Construction Protocol:	The 10X Genomics Chromium Platform was used to generate a targeted 5000 single cell Gel Bead-In-Emulsions(GEMs) per sample, loaded with an initial cell viability of ~90%. scRNA-seq libraries were prepared following the 10X Genomics user guide (Single Cell 3’Reagent Kits User Guide PN-120233, 10X Genomics).

Molecule Type:	poly(A)+ RNA
Library Source:
Library Layout:	PAIRED
Library Strand:	Forward
Platform:	ILLUMINA
Instrument Model:	Illumina HiSeq 2500
Strand-Specific:	Specific

Data Resource	GEN Sample ID	GEN Dataset ID	Project ID	BioProject ID	Sample ID	Sample Name	BioSample ID	Sample Accession	Experiment Accession	Release Date	Submission Date	Update Date	Species	Race	Ethnicity	Age	Age Unit	Gender	Source Name	Tissue	Cell Type	Cell Subtype	Cell Line	Disease	Disease State	Development Stage	Mutation	Phenotype	Case Detail	Control Detail	Growth Protocol	Treatment Protocol	Extract Protocol	Library Construction Protocol	Molecule Type	Library Layout	Strand-Specific	Library Strand	Spike-In	Strategy	Platform	Instrument Model	Cell Number	Reads Number	Gbases	AvgSpotLen1	AvgSpotLen2	Uniq Mapping Rate	Multiple Mapping Rate	Coverage Rate