概要: Safeners are an important group of agrochemicals that can extend the selective use of existing herbicides into a wider range of cereals by protecting the crops from chemical injury. The recently released aromatic sulphonamide safeners cyprosulfamide and metcamifen were tested for their ability to protect rice plants from the phytotoxicity caused by clodinafop-propargyl, a herbicide normally only used in wheat. Transcriptome studies in rice cultures demonstrated that whereas cyprosulfamide had a negligible effect on gene expression over a 4 h exposure, metcamifen perturbed the abundance of 590 transcripts. Analysis of the inducible genes suggested that safening elicited similar gene families to those associated with specific biotic and abiotic stresses, notably those elicited by abscisic acid, salicylic acid and methyl-jasmonate.
项目整体设计: Changes in the transcriptome of rice cultures exposed to metcamifen (5 µM) or cyprosulfamide (5 µM) were determined as compared with control cells treated with the carrier solvent DMSO. Cultures were sampled at 0.5 h, 1.5 h and 4 h after dosing and subjected to RNA-seq analysis. Three biological replicates were sequenced for each sample for a total of 27 samples
doesed with 5mM stokcs of metcamifen or cyprosulfamide, prepared in dimethyl fulfoxide (DMSO) to give a final concentration of 5uM, or DMSO alone as control treatmnet. Cells sampled at 30,90, and 240 minutes.
提取方案:
Total RNA was extracted using RiboPureTM RNA Purification Kit (Ambion, Inc.) Illumina Kit
