Gene Expression NebulasSummary: siRNA KD of POU3F2 directly effect DNA-damage repair and apoptosis genes
Overall Design: 501mel melanoma cell line was treated with either siCTR or siPOU3F2 prior to UVB treatment. The samples were collected at the indicated time
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| Growth Protocol: | All cell lines were grown at 37°C with 10% CO2 in RPMI-1640 (Gibco BRL, Invitrogen) supplemented with penicillin and streptomycin, 10% fetal bovine serum (FBS, Biosera). |
| Treatment Protocol: | 501mel melanoma cell line was treated with either siCTR prior to UVB treatment; 501mel melanoma cell line was treated with either siPOU3F2 prior to UVB treatment |
| Extract Protocol: | RNA-samples were extracted using QIAGEN RNeasy kit |
| Library Construction Protocol: | Library prep using QuantSeq Forward kit (LEXOGEN #015.96), using 500 ng starting material |
| Molecule Type: | poly(A)+ RNA |
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| Library Layout: | SINGLE |
| Library Strand: | Reverse |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq 4000 |
| Strand-Specific: | Specific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Condition Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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