PRJNA513348: The study of the role of POU3F2 in response to UVB treatment
概要: siRNA KD of POU3F2 directly effect DNA-damage repair and apoptosis genes
项目整体设计: 501mel melanoma cell line was treated with either siCTR or siPOU3F2 prior to UVB treatment. The samples were collected at the indicated time
| 生长方案: |
All cell lines were grown at 37°C with 10% CO2 in RPMI-1640 (Gibco BRL, Invitrogen) supplemented with penicillin and streptomycin, 10% fetal bovine serum (FBS, Biosera). |
| 处理方案: |
501mel melanoma cell line was treated with either siCTR prior to UVB treatment; 501mel melanoma cell line was treated with either siPOU3F2 prior to UVB treatment |
| 提取方案: |
RNA-samples were extracted using QIAGEN RNeasy kit |
| 建库方案: |
Library prep using QuantSeq Forward kit (LEXOGEN #015.96), using 500 ng starting material |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
SINGLE |
| 库的链类型: |
Reverse |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina HiSeq 4000 |
| 链特异性: |
Specific |
| 数据来源 |
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Condition Detail |
生长方案 |
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提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标(Spike-In) |
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平均测序片段长度_1 |
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覆盖度 |
BRN2 suppresses apoptosis, reprograms DNA damage repair, and is associated with a high somatic mutation burden in melanoma.
Genes & development . 2019-02-25 [PMID:
30804224]