Gene Expression
Nebulas
Gene Expression Nebulas概要: The Reduced Height (Rht) genes formed the basis for the green revolution in wheat by decreasing plant height and increasing productive tillers. There are two current widely used Rht mutant alleles, Rht-B1b and Rht-D1b. Both reduce plant height by 20% and increase seed yield by 5-10%. They are also associated with decreased seed size and protein content. Here we tested the degree to which Rht-B1b impacts flag leaf photosynthetic rates and carbon and nitrogen partitioning to the flag leaf and grain during grain fill under field conditions using near isogenic lines (NILs) that were either standard height (Rht-B1a) or semi-dwarf (Rht-B1b). The results demonstrate that at anthesis, Rht-B1b reduces flag leaf photosynthetic rate per unit area by 18% and chlorophyll A content by 23%. Rht-B1b significantly reduced grain protein beginning at 14 days post anthesis with the greatest difference seen at 21 days post anthesis (DPA) (12%). Rht-B1b also significantly decreased individual seed weight beginning at 21 DPA and by 15.2% at 28 DPA. Global expression analysis using RNA extracted from developing leaves and stems demonstrated that genes associated with carbon and nitrogen metabolism are not substantially altered by Rht-B1b. From this study, we conclude that Rht-B1b reduces flag leaf photosynthetic rate at flowering while changes in grain composition begin shortly after anthesis.
项目整体设计: Total RNA was collected from leaf and stem tissue from plants at 14 days past anthesis. Three samples were taken from plants carrying the semi-dwarfing allele Rht-B1b and three samples were taken from plants without the semi-dwarfing allele.
| 测序方法: |
|
| 物种: |
|
| 组织: |
|
| 高度/长度/重量: |
|
| 发育阶段: |
|
| 生长方案: | Plants were grown under field conditions near Bozeman, MT (latitude 45.6N, longitude 111.00W). Plants were irrigated with 5cm of water on week pre- and post-anthesis. |
| 处理方案: | Plants either carried the mutant semi-dwarfing allele, Rht-B1b, or the wildtype tall allele, Rht-B1a. |
| 提取方案: | Total RNA was extracted in accordance with the QIAGEN RNeasy Plant Mini kit and quantified using an Agilent Bioanalyzer. |
| 建库方案: | Libraries were prepared according to Illumina's TruSeq Stranded mRNA Library prep. |
| 分子类型: | poly(A)+ RNA |
| 库的片段类型: | SINGLE |
| 库的链类型: | Forward |
| 测序平台: | ILLUMINA |
| 测序仪型号: | Illumina HiSeq 2500 |
| 链特异性: | Specific |
| 数据来源 | GEN样本编号 | GEN数据集编号 | 系列编号 | 项目编号 | 样本编号 | 样本名称 | 生物样本编号 | 样本访问号 | 实验访问号 | 释放时间 | 提交时间 | 最后更新时间 | 物种 | 种族 | 族裔 | 年龄 | 年龄单位 | 性别 | 来源名称 | 组织 | 细胞类型 | 细胞亚型 | 细胞系 | 疾病 | 疾病状态 | 发育阶段 | 突变/变异 | 表型 | Condition Detail | 生长方案 | 处理方案 | 提取方案 | 建库方案 | 分子类型 | 库的片段类型 | 链特异性 | 库的链类型 | 加标（Spike-In） | 测序方法 | 测序平台 | 测序仪型号 | 细胞数 | 测序片段数 | 碱基数 | 平均测序片段长度_1 | 平均测序片段长度_2 | 唯一比对率 | 多重比对率 | 覆盖度 |
|---|