The hiPSC line used for deriving neurons was generated by Sendai virus method at Rutgers University Cell and DNA Repository (RUCDR)-NIMH Stem Cell Center from cryopreserved lymphocytes (CPLs) of a 29-year-old male SCZ patient (cell ID: 07C65853) from the MGS collection. The NorthShore University HealthSystem Institutional Review Board (IRB) approved the study. hiPSCs were cultured using the feeder-free method in Geltrex (Thermofisher)-coated plates in mTeSR1 medium (StemCell). The media were changed daily, and cell were passaged as clumps every 5 days using ReLeSR (StemCell) in the presence of 5 μm ROCK inhibitor (R&D Systems). hiPSCs were characterized by positive IF staining of pluripotent stem cell markers OCT4, TRA-1-60, NANOG, and SSEA4.
处理方案:
-
提取方案:
Total RNAs were extracted from homogenized cell lysate using the RNeasy Plus mini kit (Qiagen)
建库方案:
RNA libraries were prepared for sequencing using standard Illumina protocols.
测序信息
分子类型:
rRNA- RNA
库的片段类型:
PAIRED
库的链类型:
-
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 2500
链特异性:
Unspecific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源
GEN样本编号
GEN数据集编号
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样本编号
样本名称
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物种
种族
族裔
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性别
来源名称
组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
表型
Condition Detail
生长方案
处理方案
提取方案
建库方案
分子类型
库的片段类型
链特异性
库的链类型
加标（Spike-In）
测序方法
测序平台
测序仪型号
细胞数
测序片段数
碱基数
平均测序片段长度_1
平均测序片段长度_2
唯一比对率
多重比对率
覆盖度
文章
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