Project - PRJNA527782 - Gene Expression Nebulas

A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

A data portal of transcriptome profiles across multiple species

PRJNA527782: Lifecycle transcriptomics of field-droughted sorghum reveals rapid biotic and metabolic responses

Source: NCBI / GSE128441

Submission Date: Mar 18 2019

Release Date: Nov 13 2019

Update Date: Nov 13 2019

Summary: We conducted a large-scale field experiment, imposing pre- and post-flowering drought stress on two genotypes of sorghum across a tightly-resolved time series, resulting in a data set of over 350 transcriptomes root and shoot tissue.

Overall Design: RNA-seq on root and shoot tissue of Sorghum exposed to two drought regimes

GEN Datasets:

GEND000281

Strategy:	Bulk RNA-seq
Species:	Sorghum bicolor
Tissue:	Leaf Root
Isolation_source:	Frozen root and leaf tissue were ground in a cryogenic Freezer Mill (SPEX SamplePrep 6875D Metuchen NJ USA) for 2-3 cycles of 2-3 minutes with 1 minute cooling in between. Samples were then stored at -80C.
Development Stage:	105 days 112 days 119 days 14 days 21 days 28 days 35 days 42 days 49 days 56 days 63 days 70 days 77 days 84 days 91 days 98 days

Protocol

Growth Protocol:	Leaf and root samples of 2 sorghum genotypes exposed to preflowering and postflowering drought stress were collected from field-grown plants in Parlier, California.
Treatment Protocol:	RTX430 Leaves, 21 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 21 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 21 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 28 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 28 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 28 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 35 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 35 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 42 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 42 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 42 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 49 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 56 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 56 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 56 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 63 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 63 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 63 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 70 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 70 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 70 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 77 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 77 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 77 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 84 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 84 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 84 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 91 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 91 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 91 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 98 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 105 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 112 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 119 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 119 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 119 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 63 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 63 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 63 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 70 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 70 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 70 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 77 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 77 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 77 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 84 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 91 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 91 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 91 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 98 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 105 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 105 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 105 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 119 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 119 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 119 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 14 days old, Control, plot 12; RTX430 Leaves, 14 days old, Control, plot 20; RTX430 Leaves, 14 days old, Control, plot 1; RTX430 Leaves, 21 days old, Control, plot 12; RTX430 Leaves, 21 days old, Control, plot 20; RTX430 Leaves, 21 days old, Control, plot 1; RTX430 Leaves, 28 days old, Control, plot 12; RTX430 Leaves, 28 days old, Control, plot 20; RTX430 Leaves, 28 days old, Control, plot 1; RTX430 Leaves, 35 days old, Control, plot 1; RTX430 Leaves, 35 days old, Control, plot 12; RTX430 Leaves, 35 days old, Control, plot 20; RTX430 Leaves, 42 days old, Control, plot 12; RTX430 Leaves, 42 days old, Control, plot 20; RTX430 Leaves, 42 days old, Control, plot 1; RTX430 Leaves, 49 days old, Control, plot 12; RTX430 Leaves, 56 days old, Control, plot 20; RTX430 Leaves, 56 days old, Control, plot 12; RTX430 Leaves, 56 days old, Control, plot 1; RTX430 Leaves, 63 days old, Control, plot 12; RTX430 Leaves, 63 days old, Control, plot 20; RTX430 Leaves, 63 days old, Control, plot 1; RTX430 Leaves, 70 days old, Control, plot 20; RTX430 Leaves, 70 days old, Control, plot 12; RTX430 Leaves, 70 days old, Control, plot 1; RTX430 Leaves, 77 days old, Control, plot 20; RTX430 Leaves, 77 days old, Control, plot 12; RTX430 Leaves, 77 days old, Control, plot 1; RTX430 Leaves, 84 days old, Control, plot 20; RTX430 Leaves, 84 days old, Control, plot 1; RTX430 Leaves, 84 days old, Control, plot 12; RTX430 Leaves, 91 days old, Control, plot 20; RTX430 Leaves, 91 days old, Control, plot 1; RTX430 Leaves, 91 days old, Control, plot 12; RTX430 Leaves, 98 days old, Control, plot 12; RTX430 Leaves, 105 days old, Control, plot 20; RTX430 Leaves, 105 days old, Control, plot 12; RTX430 Leaves, 112 days old, Control, plot 12; RTX430 Leaves, 119 days old, Control, plot 1; RTX430 Leaves, 119 days old, Control, plot 20; RTX430 Leaves, 119 days old, Control, plot 12; BTX642 Leaves, 21 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 21 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 21 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 28 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 28 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 28 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 35 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 35 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 35 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 42 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 42 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 42 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 49 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 56 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 56 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 56 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 63 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 63 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 63 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 70 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 70 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 70 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 77 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 77 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 77 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 84 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 84 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 84 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 91 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 91 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 91 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 98 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 105 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 105 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 105 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 112 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 119 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 119 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 119 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 63 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 63 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 63 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 70 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 70 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 70 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 77 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 77 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 77 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 84 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 91 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 91 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 91 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 98 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 105 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 105 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 105 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 112 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 119 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 119 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 119 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 14 days old, Control, plot 11; BTX642 Leaves, 14 days old, Control, plot 19; BTX642 Leaves, 14 days old, Control, plot 2; BTX642 Leaves, 21 days old, Control, plot 11; BTX642 Leaves, 21 days old, Control, plot 19; BTX642 Leaves, 21 days old, Control, plot 2; BTX642 Leaves, 28 days old, Control, plot 19; BTX642 Leaves, 28 days old, Control, plot 2; BTX642 Leaves, 35 days old, Control, plot 11; BTX642 Leaves, 35 days old, Control, plot 2; BTX642 Leaves, 35 days old, Control, plot 19; BTX642 Leaves, 42 days old, Control, plot 11; BTX642 Leaves, 42 days old, Control, plot 19; BTX642 Leaves, 42 days old, Control, plot 2; BTX642 Leaves, 49 days old, Control, plot 11; BTX642 Leaves, 56 days old, Control, plot 19; BTX642 Leaves, 56 days old, Control, plot 11; BTX642 Leaves, 56 days old, Control, plot 2; BTX642 Leaves, 63 days old, Control, plot 19; BTX642 Leaves, 63 days old, Control, plot 11; BTX642 Leaves, 63 days old, Control, plot 2; BTX642 Leaves, 70 days old, Control, plot 19; BTX642 Leaves, 70 days old, Control, plot 11; BTX642 Leaves, 70 days old, Control, plot 2; BTX642 Leaves, 77 days old, Control, plot 19; BTX642 Leaves, 77 days old, Control, plot 11; BTX642 Leaves, 77 days old, Control, plot 2; BTX642 Leaves, 84 days old, Control, plot 11; BTX642 Leaves, 84 days old, Control, plot 19; BTX642 Leaves, 84 days old, Control, plot 2; BTX642 Leaves, 91 days old, Control, plot 19; BTX642 Leaves, 91 days old, Control, plot 2; BTX642 Leaves, 91 days old, Control, plot 11; BTX642 Leaves, 98 days old, Control, plot 11; BTX642 Leaves, 105 days old, Control, plot 19; BTX642 Leaves, 105 days old, Control, plot 2; BTX642 Leaves, 105 days old, Control, plot 11; BTX642 Leaves, 119 days old, Control, plot 2; BTX642 Leaves, 119 days old, Control, plot 11; BTX642 Leaves, 119 days old, Control, plot 19; RTX430 Roots, 21 days old, Preflowering drought stress, plot 5; RTX430 Roots, 21 days old, Preflowering drought stress, plot 13; RTX430 Roots, 21 days old, Preflowering drought stress, plot 18; RTX430 Roots, 28 days old, Preflowering drought stress, plot 5; RTX430 Roots, 28 days old, Preflowering drought stress, plot 13; RTX430 Roots, 28 days old, Preflowering drought stress, plot 18; RTX430 Roots, 35 days old, Preflowering drought stress, plot 13; RTX430 Roots, 35 days old, Preflowering drought stress, plot 18; RTX430 Roots, 35 days old, Preflowering drought stress, plot 5; RTX430 Roots, 42 days old, Preflowering drought stress, plot 13; RTX430 Roots, 42 days old, Preflowering drought stress, plot 5; RTX430 Roots, 42 days old, Preflowering drought stress, plot 18; RTX430 Roots, 49 days old, Preflowering drought stress, plot 5; RTX430 Roots, 56 days old, Preflowering drought stress, plot 13; RTX430 Roots, 56 days old, Preflowering drought stress, plot 5; RTX430 Roots, 56 days old, Preflowering drought stress, plot 18; RTX430 Roots, 63 days old, Preflowering drought stress, plot 5; RTX430 Roots, 63 days old, Preflowering drought stress, plot 13; RTX430 Roots, 63 days old, Preflowering drought stress, plot 18; RTX430 Roots, 70 days old, Preflowering drought stress, plot 13; RTX430 Roots, 70 days old, Preflowering drought stress, plot 5; RTX430 Roots, 70 days old, Preflowering drought stress, plot 18; RTX430 Roots, 77 days old, Preflowering drought stress, plot 13; RTX430 Roots, 77 days old, Preflowering drought stress, plot 5; RTX430 Roots, 77 days old, Preflowering drought stress, plot 18; RTX430 Roots, 84 days old, Preflowering drought stress, plot 18; RTX430 Roots, 84 days old, Preflowering drought stress, plot 13; RTX430 Roots, 84 days old, Preflowering drought stress, plot 5; RTX430 Roots, 91 days old, Preflowering drought stress, plot 13; RTX430 Roots, 91 days old, Preflowering drought stress, plot 5; RTX430 Roots, 91 days old, Preflowering drought stress, plot 18; RTX430 Roots, 98 days old, Preflowering drought stress, plot 5; RTX430 Roots, 105 days old, Preflowering drought stress, plot 5; RTX430 Roots, 105 days old, Preflowering drought stress, plot 13; RTX430 Roots, 112 days old, Preflowering drought stress, plot 5; RTX430 Roots, 119 days old, Preflowering drought stress, plot 18; RTX430 Roots, 119 days old, Preflowering drought stress, plot 13; RTX430 Roots, 119 days old, Preflowering drought stress, plot 5; RTX430 Roots, 63 days old, Postflowering drought stress, plot 3; RTX430 Roots, 63 days old, Postflowering drought stress, plot 22; RTX430 Roots, 63 days old, Postflowering drought stress, plot 16; RTX430 Roots, 70 days old, Postflowering drought stress, plot 3; RTX430 Roots, 70 days old, Postflowering drought stress, plot 22; RTX430 Roots, 70 days old, Postflowering drought stress, plot 16; RTX430 Roots, 77 days old, Postflowering drought stress, plot 3; RTX430 Roots, 77 days old, Postflowering drought stress, plot 22; RTX430 Roots, 77 days old, Postflowering drought stress, plot 16; RTX430 Roots, 84 days old, Postflowering drought stress, plot 22; RTX430 Roots, 91 days old, Postflowering drought stress, plot 3; RTX430 Roots, 91 days old, Postflowering drought stress, plot 16; RTX430 Roots, 91 days old, Postflowering drought stress, plot 22; RTX430 Roots, 98 days old, Postflowering drought stress, plot 22; RTX430 Roots, 105 days old, Postflowering drought stress, plot 3; RTX430 Roots, 105 days old, Postflowering drought stress, plot 16; RTX430 Roots, 105 days old, Postflowering drought stress, plot 22; RTX430 Roots, 112 days old, Postflowering drought stress, plot 22; RTX430 Roots, 119 days old, Postflowering drought stress, plot 22; RTX430 Roots, 119 days old, Postflowering drought stress, plot 3; RTX430 Roots, 119 days old, Postflowering drought stress, plot 16; RTX430 Roots, 14 days old, Control, plot 12; RTX430 Roots, 14 days old, Control, plot 20; RTX430 Roots, 14 days old, Control, plot 1; RTX430 Roots, 21 days old, Control, plot 12; RTX430 Roots, 21 days old, Control, plot 20; RTX430 Roots, 21 days old, Control, plot 1; RTX430 Roots, 28 days old, Control, plot 12; RTX430 Roots, 28 days old, Control, plot 20; RTX430 Roots, 28 days old, Control, plot 1; RTX430 Roots, 35 days old, Control, plot 1; RTX430 Roots, 35 days old, Control, plot 20; RTX430 Roots, 35 days old, Control, plot 12; RTX430 Roots, 42 days old, Control, plot 12; RTX430 Roots, 42 days old, Control, plot 20; RTX430 Roots, 42 days old, Control, plot 1; RTX430 Roots, 49 days old, Control, plot 12; RTX430 Roots, 56 days old, Control, plot 12; RTX430 Roots, 56 days old, Control, plot 20; RTX430 Roots, 56 days old, Control, plot 1; RTX430 Roots, 63 days old, Control, plot 20; RTX430 Roots, 63 days old, Control, plot 12; RTX430 Roots, 63 days old, Control, plot 1; RTX430 Roots, 70 days old, Control, plot 20; RTX430 Roots, 70 days old, Control, plot 12; RTX430 Roots, 70 days old, Control, plot 1; RTX430 Roots, 77 days old, Control, plot 20; RTX430 Roots, 77 days old, Control, plot 12; RTX430 Roots, 77 days old, Control, plot 1; RTX430 Roots, 84 days old, Control, plot 20; RTX430 Roots, 84 days old, Control, plot 12; RTX430 Roots, 91 days old, Control, plot 20; RTX430 Roots, 91 days old, Control, plot 1; RTX430 Roots, 91 days old, Control, plot 12; RTX430 Roots, 98 days old, Control, plot 12; RTX430 Roots, 105 days old, Control, plot 12; RTX430 Roots, 105 days old, Control, plot 20; RTX430 Roots, 105 days old, Control, plot 1; RTX430 Roots, 112 days old, Control, plot 12; RTX430 Roots, 119 days old, Control, plot 20; RTX430 Roots, 119 days old, Control, plot 1; RTX430 Roots, 119 days old, Control, plot 12; BTX642 Roots, 21 days old, Preflowering drought stress, plot 6; BTX642 Roots, 21 days old, Preflowering drought stress, plot 14; BTX642 Roots, 21 days old, Preflowering drought stress, plot 17; BTX642 Roots, 28 days old, Preflowering drought stress, plot 6; BTX642 Roots, 28 days old, Preflowering drought stress, plot 14; BTX642 Roots, 28 days old, Preflowering drought stress, plot 17; BTX642 Roots, 35 days old, Preflowering drought stress, plot 6; BTX642 Roots, 35 days old, Preflowering drought stress, plot 14; BTX642 Roots, 35 days old, Preflowering drought stress, plot 17; BTX642 Roots, 42 days old, Preflowering drought stress, plot 6; BTX642 Roots, 42 days old, Preflowering drought stress, plot 14; BTX642 Roots, 42 days old, Preflowering drought stress, plot 17; BTX642 Roots, 49 days old, Preflowering drought stress, plot 6; BTX642 Roots, 56 days old, Preflowering drought stress, plot 6; BTX642 Roots, 56 days old, Preflowering drought stress, plot 17; BTX642 Roots, 63 days old, Preflowering drought stress, plot 14; BTX642 Roots, 63 days old, Preflowering drought stress, plot 6; BTX642 Roots, 63 days old, Preflowering drought stress, plot 17; BTX642 Roots, 70 days old, Preflowering drought stress, plot 14; BTX642 Roots, 70 days old, Preflowering drought stress, plot 6; BTX642 Roots, 70 days old, Preflowering drought stress, plot 17; BTX642 Roots, 77 days old, Preflowering drought stress, plot 14; BTX642 Roots, 77 days old, Preflowering drought stress, plot 6; BTX642 Roots, 77 days old, Preflowering drought stress, plot 17; BTX642 Roots, 84 days old, Preflowering drought stress, plot 6; BTX642 Roots, 84 days old, Preflowering drought stress, plot 14; BTX642 Roots, 84 days old, Preflowering drought stress, plot 17; BTX642 Roots, 91 days old, Preflowering drought stress, plot 14; BTX642 Roots, 91 days old, Preflowering drought stress, plot 17; BTX642 Roots, 91 days old, Preflowering drought stress, plot 6; BTX642 Roots, 98 days old, Preflowering drought stress, plot 6; BTX642 Roots, 105 days old, Preflowering drought stress, plot 14; BTX642 Roots, 105 days old, Preflowering drought stress, plot 17; BTX642 Roots, 112 days old, Preflowering drought stress, plot 6; BTX642 Roots, 119 days old, Preflowering drought stress, plot 6; BTX642 Roots, 119 days old, Preflowering drought stress, plot 14; BTX642 Roots, 119 days old, Preflowering drought stress, plot 17; BTX642 Roots, 63 days old, Postflowering drought stress, plot 4; BTX642 Roots, 63 days old, Postflowering drought stress, plot 21; BTX642 Roots, 63 days old, Postflowering drought stress, plot 15; BTX642 Roots, 70 days old, Postflowering drought stress, plot 4; BTX642 Roots, 70 days old, Postflowering drought stress, plot 21; BTX642 Roots, 70 days old, Postflowering drought stress, plot 15; BTX642 Roots, 77 days old, Postflowering drought stress, plot 4; BTX642 Roots, 77 days old, Postflowering drought stress, plot 21; BTX642 Roots, 77 days old, Postflowering drought stress, plot 15; BTX642 Roots, 84 days old, Postflowering drought stress, plot 21; BTX642 Roots, 91 days old, Postflowering drought stress, plot 4; BTX642 Roots, 91 days old, Postflowering drought stress, plot 15; BTX642 Roots, 91 days old, Postflowering drought stress, plot 21; BTX642 Roots, 98 days old, Postflowering drought stress, plot 21; BTX642 Roots, 105 days old, Postflowering drought stress, plot 15; BTX642 Roots, 105 days old, Postflowering drought stress, plot 21; BTX642 Roots, 105 days old, Postflowering drought stress, plot 4; BTX642 Roots, 112 days old, Postflowering drought stress, plot 21; BTX642 Roots, 119 days old, Postflowering drought stress, plot 4; BTX642 Roots, 119 days old, Postflowering drought stress, plot 15; BTX642 Roots, 119 days old, Postflowering drought stress, plot 21; BTX642 Roots, 14 days old, Control, plot 11; BTX642 Roots, 14 days old, Control, plot 19; BTX642 Roots, 14 days old, Control, plot 2; BTX642 Roots, 21 days old, Control, plot 11; BTX642 Roots, 21 days old, Control, plot 19; BTX642 Roots, 21 days old, Control, plot 2; BTX642 Roots, 28 days old, Control, plot 11; BTX642 Roots, 28 days old, Control, plot 19; BTX642 Roots, 28 days old, Control, plot 2; BTX642 Roots, 35 days old, Control, plot 11; BTX642 Roots, 35 days old, Control, plot 2; BTX642 Roots, 35 days old, Control, plot 19; BTX642 Roots, 42 days old, Control, plot 11; BTX642 Roots, 42 days old, Control, plot 19; BTX642 Roots, 42 days old, Control, plot 2; BTX642 Roots, 49 days old, Control, plot 11; BTX642 Roots, 56 days old, Control, plot 19; BTX642 Roots, 56 days old, Control, plot 11; BTX642 Roots, 56 days old, Control, plot 2; BTX642 Roots, 63 days old, Control, plot 19; BTX642 Roots, 63 days old, Control, plot 2; BTX642 Roots, 63 days old, Control, plot 11; BTX642 Roots, 70 days old, Control, plot 19; BTX642 Roots, 70 days old, Control, plot 11; BTX642 Roots, 70 days old, Control, plot 2; BTX642 Roots, 77 days old, Control, plot 19; BTX642 Roots, 77 days old, Control, plot 11; BTX642 Roots, 77 days old, Control, plot 2; BTX642 Roots, 84 days old, Control, plot 11; BTX642 Roots, 84 days old, Control, plot 2; BTX642 Roots, 84 days old, Control, plot 19; BTX642 Roots, 91 days old, Control, plot 19; BTX642 Roots, 91 days old, Control, plot 2; BTX642 Roots, 91 days old, Control, plot 11; BTX642 Roots, 98 days old, Control, plot 11; BTX642 Roots, 105 days old, Control, plot 19; BTX642 Roots, 105 days old, Control, plot 11; BTX642 Roots, 119 days old, Control, plot 11; BTX642 Roots, 119 days old, Control, plot 19; BTX642 Roots, 119 days old, Control, plot 2
Extract Protocol:	Frozen root and leaf tissue were ground in a cryogenic Freezer Mill (SPEX SamplePrep 6875D, Metuchen NJ USA) for 2-3 cycles of 2-3 minutes, with 1 minute cooling in between. Samples were then stored at -80C. RNA was extracted using the QIAGEN miRNeasy mini kit (Cat. #AM217004) with modifications: 1 mL QIAzol (Cat) and 100 uL 10% Sarkosyl solution were added to 100 mg of frozen tissue and placed in a vortex shaker adapter for 5 minutes. To these, 200 uL chloroform was added, and the samples were then vortexed and incubated for 3 minutes at room temperature before centrifugation at 12,000 xg for 15 min at 4C. The aqueous phase was then transferred to a fresh tube, and ethanol was added to a final concentration of 60%. The remaining procedure was performed according to the manufacturer’s recommendations. DNA contamination was removed from each sample using the TURBO DNA-free kit, (Cat. # AM1907, Invitrogen) according to the manufacturer’s recommendations.
Library Construction Protocol:	Stranded cDNA libraries were generated using the Illumina Truseq Stranded RNA LT kit. mRNA was purified from 1 ug of total RNA using magnetic beads containing poly-T oligos. mRNA was fragmented and reversed transcribed using random hexamers and SSII (Invitrogen) followed by second strand synthesis. The fragmented cDNA was treated with end-pair, A-tailing, adapter ligation, and 8 cycles of PCR. qPCR was used to determine the concentration of the libraries.

Sequencing

Molecule Type:	poly(A)+ RNA
Library Source:
Library Layout:	SINGLE; PAIRED
Library Strand:	Forward
Platform:	ILLUMINA
Instrument Model:	Illumina HiSeq 2500; Illumina HiSeq 2000
Strand-Specific:	Specific

Samples

Biological Condition:

Disease

Disease State

Development Stage

Mutation

Phenotype

Experimental Variables:

Case Detail

Control Detail

Protocol:

Growth Protocol

Treatment Protocol

Extract Protocol

Library Construction Protocol

Molecule Type

Library Layout

Strand-Specific

Library Strand

Spike-in

Sequencing:

Strategy

Platform

Instrument Model

Assessing Quality:

Cell Number

Reads Number

Gbases

AvgSpotLen1

AvgSpotLen2

Unique-Mapping Rate

Multi-Mapping Rate

Coverage Rate

Analysis:

Reference Genome

Genome Annotation

Data Processing

Data Resource	GEN Sample ID	GEN Dataset ID	Project ID	BioProject ID	Sample ID	Sample Name	BioSample ID	Sample Accession	Experiment Accession	Release Date	Submission Date	Update Date	Species	Race	Ethnicity	Age	Age Unit	Gender	Source Name	Tissue	Cell Type	Cell Subtype	Cell Line	Disease	Disease State	Development Stage	Mutation	Phenotype	Case Detail	Control Detail	Growth Protocol	Treatment Protocol	Extract Protocol	Library Construction Protocol	Molecule Type	Library Layout	Strand-Specific	Library Strand	Spike-In	Strategy	Platform	Instrument Model	Cell Number	Reads Number	Gbases	AvgSpotLen1	AvgSpotLen2	Uniq Mapping Rate	Multiple Mapping Rate	Coverage Rate

Publications

Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses.

Proceedings of the National Academy of Sciences of the United States of America . 2019-12-05 [PMID: 31806758]