Gene Expression Nebulas
基于标准化流程分析的转录图谱综合数据库

Gene Expression Nebulas

多物种转录图谱整合数据库

PRJNA527782: Lifecycle transcriptomics of field-droughted sorghum reveals rapid biotic and metabolic responses

来源: NCBI / GSE128441
提交时间: Mar 18 2019
释放时间: Nov 13 2019
最后更新时间: Nov 13 2019

概要: We conducted a large-scale field experiment, imposing pre- and post-flowering drought stress on two genotypes of sorghum across a tightly-resolved time series, resulting in a data set of over 350 transcriptomes root and shoot tissue.

项目整体设计: RNA-seq on root and shoot tissue of Sorghum exposed to two drought regimes

GEN 数据集:
GEND000281
测序方法:
物种:
组织:
分离条件:
发育阶段:
方案
生长方案: Leaf and root samples of 2 sorghum genotypes exposed to preflowering and postflowering drought stress were collected from field-grown plants in Parlier, California.
处理方案: RTX430 Leaves, 21 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 21 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 21 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 28 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 28 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 28 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 35 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 35 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 42 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 42 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 42 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 49 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 56 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 56 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 56 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 63 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 63 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 63 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 70 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 70 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 70 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 77 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 77 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 77 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 84 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 84 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 84 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 91 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 91 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 91 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 98 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 105 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 112 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 119 days old, Preflowering drought stress, plot 13; RTX430 Leaves, 119 days old, Preflowering drought stress, plot 5; RTX430 Leaves, 119 days old, Preflowering drought stress, plot 18; RTX430 Leaves, 63 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 63 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 63 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 70 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 70 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 70 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 77 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 77 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 77 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 84 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 91 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 91 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 91 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 98 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 105 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 105 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 105 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 119 days old, Postflowering drought stress, plot 3; RTX430 Leaves, 119 days old, Postflowering drought stress, plot 16; RTX430 Leaves, 119 days old, Postflowering drought stress, plot 22; RTX430 Leaves, 14 days old, Control, plot 12; RTX430 Leaves, 14 days old, Control, plot 20; RTX430 Leaves, 14 days old, Control, plot 1; RTX430 Leaves, 21 days old, Control, plot 12; RTX430 Leaves, 21 days old, Control, plot 20; RTX430 Leaves, 21 days old, Control, plot 1; RTX430 Leaves, 28 days old, Control, plot 12; RTX430 Leaves, 28 days old, Control, plot 20; RTX430 Leaves, 28 days old, Control, plot 1; RTX430 Leaves, 35 days old, Control, plot 1; RTX430 Leaves, 35 days old, Control, plot 12; RTX430 Leaves, 35 days old, Control, plot 20; RTX430 Leaves, 42 days old, Control, plot 12; RTX430 Leaves, 42 days old, Control, plot 20; RTX430 Leaves, 42 days old, Control, plot 1; RTX430 Leaves, 49 days old, Control, plot 12; RTX430 Leaves, 56 days old, Control, plot 20; RTX430 Leaves, 56 days old, Control, plot 12; RTX430 Leaves, 56 days old, Control, plot 1; RTX430 Leaves, 63 days old, Control, plot 12; RTX430 Leaves, 63 days old, Control, plot 20; RTX430 Leaves, 63 days old, Control, plot 1; RTX430 Leaves, 70 days old, Control, plot 20; RTX430 Leaves, 70 days old, Control, plot 12; RTX430 Leaves, 70 days old, Control, plot 1; RTX430 Leaves, 77 days old, Control, plot 20; RTX430 Leaves, 77 days old, Control, plot 12; RTX430 Leaves, 77 days old, Control, plot 1; RTX430 Leaves, 84 days old, Control, plot 20; RTX430 Leaves, 84 days old, Control, plot 1; RTX430 Leaves, 84 days old, Control, plot 12; RTX430 Leaves, 91 days old, Control, plot 20; RTX430 Leaves, 91 days old, Control, plot 1; RTX430 Leaves, 91 days old, Control, plot 12; RTX430 Leaves, 98 days old, Control, plot 12; RTX430 Leaves, 105 days old, Control, plot 20; RTX430 Leaves, 105 days old, Control, plot 12; RTX430 Leaves, 112 days old, Control, plot 12; RTX430 Leaves, 119 days old, Control, plot 1; RTX430 Leaves, 119 days old, Control, plot 20; RTX430 Leaves, 119 days old, Control, plot 12; BTX642 Leaves, 21 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 21 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 21 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 28 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 28 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 28 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 35 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 35 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 35 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 42 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 42 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 42 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 49 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 56 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 56 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 56 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 63 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 63 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 63 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 70 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 70 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 70 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 77 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 77 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 77 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 84 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 84 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 84 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 91 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 91 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 91 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 98 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 105 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 105 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 105 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 112 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 119 days old, Preflowering drought stress, plot 6; BTX642 Leaves, 119 days old, Preflowering drought stress, plot 14; BTX642 Leaves, 119 days old, Preflowering drought stress, plot 17; BTX642 Leaves, 63 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 63 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 63 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 70 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 70 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 70 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 77 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 77 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 77 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 84 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 91 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 91 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 91 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 98 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 105 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 105 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 105 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 112 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 119 days old, Postflowering drought stress, plot 4; BTX642 Leaves, 119 days old, Postflowering drought stress, plot 15; BTX642 Leaves, 119 days old, Postflowering drought stress, plot 21; BTX642 Leaves, 14 days old, Control, plot 11; BTX642 Leaves, 14 days old, Control, plot 19; BTX642 Leaves, 14 days old, Control, plot 2; BTX642 Leaves, 21 days old, Control, plot 11; BTX642 Leaves, 21 days old, Control, plot 19; BTX642 Leaves, 21 days old, Control, plot 2; BTX642 Leaves, 28 days old, Control, plot 19; BTX642 Leaves, 28 days old, Control, plot 2; BTX642 Leaves, 35 days old, Control, plot 11; BTX642 Leaves, 35 days old, Control, plot 2; BTX642 Leaves, 35 days old, Control, plot 19; BTX642 Leaves, 42 days old, Control, plot 11; BTX642 Leaves, 42 days old, Control, plot 19; BTX642 Leaves, 42 days old, Control, plot 2; BTX642 Leaves, 49 days old, Control, plot 11; BTX642 Leaves, 56 days old, Control, plot 19; BTX642 Leaves, 56 days old, Control, plot 11; BTX642 Leaves, 56 days old, Control, plot 2; BTX642 Leaves, 63 days old, Control, plot 19; BTX642 Leaves, 63 days old, Control, plot 11; BTX642 Leaves, 63 days old, Control, plot 2; BTX642 Leaves, 70 days old, Control, plot 19; BTX642 Leaves, 70 days old, Control, plot 11; BTX642 Leaves, 70 days old, Control, plot 2; BTX642 Leaves, 77 days old, Control, plot 19; BTX642 Leaves, 77 days old, Control, plot 11; BTX642 Leaves, 77 days old, Control, plot 2; BTX642 Leaves, 84 days old, Control, plot 11; BTX642 Leaves, 84 days old, Control, plot 19; BTX642 Leaves, 84 days old, Control, plot 2; BTX642 Leaves, 91 days old, Control, plot 19; BTX642 Leaves, 91 days old, Control, plot 2; BTX642 Leaves, 91 days old, Control, plot 11; BTX642 Leaves, 98 days old, Control, plot 11; BTX642 Leaves, 105 days old, Control, plot 19; BTX642 Leaves, 105 days old, Control, plot 2; BTX642 Leaves, 105 days old, Control, plot 11; BTX642 Leaves, 119 days old, Control, plot 2; BTX642 Leaves, 119 days old, Control, plot 11; BTX642 Leaves, 119 days old, Control, plot 19; RTX430 Roots, 21 days old, Preflowering drought stress, plot 5; RTX430 Roots, 21 days old, Preflowering drought stress, plot 13; RTX430 Roots, 21 days old, Preflowering drought stress, plot 18; RTX430 Roots, 28 days old, Preflowering drought stress, plot 5; RTX430 Roots, 28 days old, Preflowering drought stress, plot 13; RTX430 Roots, 28 days old, Preflowering drought stress, plot 18; RTX430 Roots, 35 days old, Preflowering drought stress, plot 13; RTX430 Roots, 35 days old, Preflowering drought stress, plot 18; RTX430 Roots, 35 days old, Preflowering drought stress, plot 5; RTX430 Roots, 42 days old, Preflowering drought stress, plot 13; RTX430 Roots, 42 days old, Preflowering drought stress, plot 5; RTX430 Roots, 42 days old, Preflowering drought stress, plot 18; RTX430 Roots, 49 days old, Preflowering drought stress, plot 5; RTX430 Roots, 56 days old, Preflowering drought stress, plot 13; RTX430 Roots, 56 days old, Preflowering drought stress, plot 5; RTX430 Roots, 56 days old, Preflowering drought stress, plot 18; RTX430 Roots, 63 days old, Preflowering drought stress, plot 5; RTX430 Roots, 63 days old, Preflowering drought stress, plot 13; RTX430 Roots, 63 days old, Preflowering drought stress, plot 18; RTX430 Roots, 70 days old, Preflowering drought stress, plot 13; RTX430 Roots, 70 days old, Preflowering drought stress, plot 5; RTX430 Roots, 70 days old, Preflowering drought stress, plot 18; RTX430 Roots, 77 days old, Preflowering drought stress, plot 13; RTX430 Roots, 77 days old, Preflowering drought stress, plot 5; RTX430 Roots, 77 days old, Preflowering drought stress, plot 18; RTX430 Roots, 84 days old, Preflowering drought stress, plot 18; RTX430 Roots, 84 days old, Preflowering drought stress, plot 13; RTX430 Roots, 84 days old, Preflowering drought stress, plot 5; RTX430 Roots, 91 days old, Preflowering drought stress, plot 13; RTX430 Roots, 91 days old, Preflowering drought stress, plot 5; RTX430 Roots, 91 days old, Preflowering drought stress, plot 18; RTX430 Roots, 98 days old, Preflowering drought stress, plot 5; RTX430 Roots, 105 days old, Preflowering drought stress, plot 5; RTX430 Roots, 105 days old, Preflowering drought stress, plot 13; RTX430 Roots, 112 days old, Preflowering drought stress, plot 5; RTX430 Roots, 119 days old, Preflowering drought stress, plot 18; RTX430 Roots, 119 days old, Preflowering drought stress, plot 13; RTX430 Roots, 119 days old, Preflowering drought stress, plot 5; RTX430 Roots, 63 days old, Postflowering drought stress, plot 3; RTX430 Roots, 63 days old, Postflowering drought stress, plot 22; RTX430 Roots, 63 days old, Postflowering drought stress, plot 16; RTX430 Roots, 70 days old, Postflowering drought stress, plot 3; RTX430 Roots, 70 days old, Postflowering drought stress, plot 22; RTX430 Roots, 70 days old, Postflowering drought stress, plot 16; RTX430 Roots, 77 days old, Postflowering drought stress, plot 3; RTX430 Roots, 77 days old, Postflowering drought stress, plot 22; RTX430 Roots, 77 days old, Postflowering drought stress, plot 16; RTX430 Roots, 84 days old, Postflowering drought stress, plot 22; RTX430 Roots, 91 days old, Postflowering drought stress, plot 3; RTX430 Roots, 91 days old, Postflowering drought stress, plot 16; RTX430 Roots, 91 days old, Postflowering drought stress, plot 22; RTX430 Roots, 98 days old, Postflowering drought stress, plot 22; RTX430 Roots, 105 days old, Postflowering drought stress, plot 3; RTX430 Roots, 105 days old, Postflowering drought stress, plot 16; RTX430 Roots, 105 days old, Postflowering drought stress, plot 22; RTX430 Roots, 112 days old, Postflowering drought stress, plot 22; RTX430 Roots, 119 days old, Postflowering drought stress, plot 22; RTX430 Roots, 119 days old, Postflowering drought stress, plot 3; RTX430 Roots, 119 days old, Postflowering drought stress, plot 16; RTX430 Roots, 14 days old, Control, plot 12; RTX430 Roots, 14 days old, Control, plot 20; RTX430 Roots, 14 days old, Control, plot 1; RTX430 Roots, 21 days old, Control, plot 12; RTX430 Roots, 21 days old, Control, plot 20; RTX430 Roots, 21 days old, Control, plot 1; RTX430 Roots, 28 days old, Control, plot 12; RTX430 Roots, 28 days old, Control, plot 20; RTX430 Roots, 28 days old, Control, plot 1; RTX430 Roots, 35 days old, Control, plot 1; RTX430 Roots, 35 days old, Control, plot 20; RTX430 Roots, 35 days old, Control, plot 12; RTX430 Roots, 42 days old, Control, plot 12; RTX430 Roots, 42 days old, Control, plot 20; RTX430 Roots, 42 days old, Control, plot 1; RTX430 Roots, 49 days old, Control, plot 12; RTX430 Roots, 56 days old, Control, plot 12; RTX430 Roots, 56 days old, Control, plot 20; RTX430 Roots, 56 days old, Control, plot 1; RTX430 Roots, 63 days old, Control, plot 20; RTX430 Roots, 63 days old, Control, plot 12; RTX430 Roots, 63 days old, Control, plot 1; RTX430 Roots, 70 days old, Control, plot 20; RTX430 Roots, 70 days old, Control, plot 12; RTX430 Roots, 70 days old, Control, plot 1; RTX430 Roots, 77 days old, Control, plot 20; RTX430 Roots, 77 days old, Control, plot 12; RTX430 Roots, 77 days old, Control, plot 1; RTX430 Roots, 84 days old, Control, plot 20; RTX430 Roots, 84 days old, Control, plot 12; RTX430 Roots, 91 days old, Control, plot 20; RTX430 Roots, 91 days old, Control, plot 1; RTX430 Roots, 91 days old, Control, plot 12; RTX430 Roots, 98 days old, Control, plot 12; RTX430 Roots, 105 days old, Control, plot 12; RTX430 Roots, 105 days old, Control, plot 20; RTX430 Roots, 105 days old, Control, plot 1; RTX430 Roots, 112 days old, Control, plot 12; RTX430 Roots, 119 days old, Control, plot 20; RTX430 Roots, 119 days old, Control, plot 1; RTX430 Roots, 119 days old, Control, plot 12; BTX642 Roots, 21 days old, Preflowering drought stress, plot 6; BTX642 Roots, 21 days old, Preflowering drought stress, plot 14; BTX642 Roots, 21 days old, Preflowering drought stress, plot 17; BTX642 Roots, 28 days old, Preflowering drought stress, plot 6; BTX642 Roots, 28 days old, Preflowering drought stress, plot 14; BTX642 Roots, 28 days old, Preflowering drought stress, plot 17; BTX642 Roots, 35 days old, Preflowering drought stress, plot 6; BTX642 Roots, 35 days old, Preflowering drought stress, plot 14; BTX642 Roots, 35 days old, Preflowering drought stress, plot 17; BTX642 Roots, 42 days old, Preflowering drought stress, plot 6; BTX642 Roots, 42 days old, Preflowering drought stress, plot 14; BTX642 Roots, 42 days old, Preflowering drought stress, plot 17; BTX642 Roots, 49 days old, Preflowering drought stress, plot 6; BTX642 Roots, 56 days old, Preflowering drought stress, plot 6; BTX642 Roots, 56 days old, Preflowering drought stress, plot 17; BTX642 Roots, 63 days old, Preflowering drought stress, plot 14; BTX642 Roots, 63 days old, Preflowering drought stress, plot 6; BTX642 Roots, 63 days old, Preflowering drought stress, plot 17; BTX642 Roots, 70 days old, Preflowering drought stress, plot 14; BTX642 Roots, 70 days old, Preflowering drought stress, plot 6; BTX642 Roots, 70 days old, Preflowering drought stress, plot 17; BTX642 Roots, 77 days old, Preflowering drought stress, plot 14; BTX642 Roots, 77 days old, Preflowering drought stress, plot 6; BTX642 Roots, 77 days old, Preflowering drought stress, plot 17; BTX642 Roots, 84 days old, Preflowering drought stress, plot 6; BTX642 Roots, 84 days old, Preflowering drought stress, plot 14; BTX642 Roots, 84 days old, Preflowering drought stress, plot 17; BTX642 Roots, 91 days old, Preflowering drought stress, plot 14; BTX642 Roots, 91 days old, Preflowering drought stress, plot 17; BTX642 Roots, 91 days old, Preflowering drought stress, plot 6; BTX642 Roots, 98 days old, Preflowering drought stress, plot 6; BTX642 Roots, 105 days old, Preflowering drought stress, plot 14; BTX642 Roots, 105 days old, Preflowering drought stress, plot 17; BTX642 Roots, 112 days old, Preflowering drought stress, plot 6; BTX642 Roots, 119 days old, Preflowering drought stress, plot 6; BTX642 Roots, 119 days old, Preflowering drought stress, plot 14; BTX642 Roots, 119 days old, Preflowering drought stress, plot 17; BTX642 Roots, 63 days old, Postflowering drought stress, plot 4; BTX642 Roots, 63 days old, Postflowering drought stress, plot 21; BTX642 Roots, 63 days old, Postflowering drought stress, plot 15; BTX642 Roots, 70 days old, Postflowering drought stress, plot 4; BTX642 Roots, 70 days old, Postflowering drought stress, plot 21; BTX642 Roots, 70 days old, Postflowering drought stress, plot 15; BTX642 Roots, 77 days old, Postflowering drought stress, plot 4; BTX642 Roots, 77 days old, Postflowering drought stress, plot 21; BTX642 Roots, 77 days old, Postflowering drought stress, plot 15; BTX642 Roots, 84 days old, Postflowering drought stress, plot 21; BTX642 Roots, 91 days old, Postflowering drought stress, plot 4; BTX642 Roots, 91 days old, Postflowering drought stress, plot 15; BTX642 Roots, 91 days old, Postflowering drought stress, plot 21; BTX642 Roots, 98 days old, Postflowering drought stress, plot 21; BTX642 Roots, 105 days old, Postflowering drought stress, plot 15; BTX642 Roots, 105 days old, Postflowering drought stress, plot 21; BTX642 Roots, 105 days old, Postflowering drought stress, plot 4; BTX642 Roots, 112 days old, Postflowering drought stress, plot 21; BTX642 Roots, 119 days old, Postflowering drought stress, plot 4; BTX642 Roots, 119 days old, Postflowering drought stress, plot 15; BTX642 Roots, 119 days old, Postflowering drought stress, plot 21; BTX642 Roots, 14 days old, Control, plot 11; BTX642 Roots, 14 days old, Control, plot 19; BTX642 Roots, 14 days old, Control, plot 2; BTX642 Roots, 21 days old, Control, plot 11; BTX642 Roots, 21 days old, Control, plot 19; BTX642 Roots, 21 days old, Control, plot 2; BTX642 Roots, 28 days old, Control, plot 11; BTX642 Roots, 28 days old, Control, plot 19; BTX642 Roots, 28 days old, Control, plot 2; BTX642 Roots, 35 days old, Control, plot 11; BTX642 Roots, 35 days old, Control, plot 2; BTX642 Roots, 35 days old, Control, plot 19; BTX642 Roots, 42 days old, Control, plot 11; BTX642 Roots, 42 days old, Control, plot 19; BTX642 Roots, 42 days old, Control, plot 2; BTX642 Roots, 49 days old, Control, plot 11; BTX642 Roots, 56 days old, Control, plot 19; BTX642 Roots, 56 days old, Control, plot 11; BTX642 Roots, 56 days old, Control, plot 2; BTX642 Roots, 63 days old, Control, plot 19; BTX642 Roots, 63 days old, Control, plot 2; BTX642 Roots, 63 days old, Control, plot 11; BTX642 Roots, 70 days old, Control, plot 19; BTX642 Roots, 70 days old, Control, plot 11; BTX642 Roots, 70 days old, Control, plot 2; BTX642 Roots, 77 days old, Control, plot 19; BTX642 Roots, 77 days old, Control, plot 11; BTX642 Roots, 77 days old, Control, plot 2; BTX642 Roots, 84 days old, Control, plot 11; BTX642 Roots, 84 days old, Control, plot 2; BTX642 Roots, 84 days old, Control, plot 19; BTX642 Roots, 91 days old, Control, plot 19; BTX642 Roots, 91 days old, Control, plot 2; BTX642 Roots, 91 days old, Control, plot 11; BTX642 Roots, 98 days old, Control, plot 11; BTX642 Roots, 105 days old, Control, plot 19; BTX642 Roots, 105 days old, Control, plot 11; BTX642 Roots, 119 days old, Control, plot 11; BTX642 Roots, 119 days old, Control, plot 19; BTX642 Roots, 119 days old, Control, plot 2
提取方案: Frozen root and leaf tissue were ground in a cryogenic Freezer Mill (SPEX SamplePrep 6875D, Metuchen NJ USA) for 2-3 cycles of 2-3 minutes, with 1 minute cooling in between. Samples were then stored at -80C. RNA was extracted using the QIAGEN miRNeasy mini kit (Cat. #AM217004) with modifications: 1 mL QIAzol (Cat) and 100 uL 10% Sarkosyl solution were added to 100 mg of frozen tissue and placed in a vortex shaker adapter for 5 minutes. To these, 200 uL chloroform was added, and the samples were then vortexed and incubated for 3 minutes at room temperature before centrifugation at 12,000 xg for 15 min at 4C. The aqueous phase was then transferred to a fresh tube, and ethanol was added to a final concentration of 60%. The remaining procedure was performed according to the manufacturer’s recommendations. DNA contamination was removed from each sample using the TURBO DNA-free kit, (Cat. # AM1907, Invitrogen) according to the manufacturer’s recommendations.
建库方案: Stranded cDNA libraries were generated using the Illumina Truseq Stranded RNA LT kit. mRNA was purified from 1 ug of total RNA using magnetic beads containing poly-T oligos. mRNA was fragmented and reversed transcribed using random hexamers and SSII (Invitrogen) followed by second strand synthesis. The fragmented cDNA was treated with end-pair, A-tailing, adapter ligation, and 8 cycles of PCR. qPCR was used to determine the concentration of the libraries.
测序信息
分子类型: poly(A)+ RNA
库的片段类型: SINGLE; PAIRED
库的链类型: Forward
测序平台: ILLUMINA
测序仪型号: Illumina HiSeq 2500; Illumina HiSeq 2000
链特异性: Specific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源 GEN样本编号 GEN数据集编号 系列编号 项目编号 样本编号 样本名称 生物样本编号 样本访问号 实验访问号 释放时间 提交时间 最后更新时间 物种 种族 族裔 年龄 年龄单位 性别 来源名称 组织 细胞类型 细胞亚型 细胞系 疾病 疾病状态 发育阶段 突变/变异 表型 Condition Detail 生长方案 处理方案 提取方案 建库方案 分子类型 库的片段类型 链特异性 库的链类型 加标(Spike-In) 测序方法 测序平台 测序仪型号 细胞数 测序片段数 碱基数 平均测序片段长度_1 平均测序片段长度_2 唯一比对率 多重比对率 覆盖度
文章
Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses.
Proceedings of the National Academy of Sciences of the United States of America . 2019-12-05 [PMID: 31806758]