Gene Expression Nebulas
基于标准化流程分析的转录图谱综合数据库

Gene Expression Nebulas

多物种转录图谱整合数据库

PRJNA533606: Single cell RNA-Seq from bone marrow Ewing patients

来源: NCBI / GSE130020
提交时间: Apr 18 2010
释放时间: Feb 14 2020
最后更新时间: Feb 14 2020

概要: We report the application of single-molecule-based sequencing technology for high-throughput profiling of bone marrow Ewing patients

项目整体设计: Study of transcriptomic heterogeneity of Ewing Sarcoma models and comparison with control cell models

GEN 数据集:
GEND000063
测序方法:
物种:
健康状况:
细胞类型:
细胞系:
方案
生长方案: MSCs from bone marrow Ewing patients were isolated by density-gradient centrifugation using Ficoll technique and were cultured in alpha MEM (Gibco), MSC-qualified serum (Gibco), 1% L-glutamine (Gibco) and 1 ng/mL bFGF (Sigma), added ex-tempo. CLB-berlud are cultured in RPMI (Gibco) 10% FBS (Eurobio), 100U/mL.
处理方案: No treatment
提取方案: Dissociated cells were captured and processed with the C1 Single-Cell Auto Prep System (FluidigmTM) following the manufacturer' protocol
建库方案: After harvest from the C1 device, each cDNA library was tagmented using the Nextera XT DNA Sample Preparation Kit (Illumina). Libraries were quantified by Qubit fluorometric assay (Invitrogen) with dsDNA HS (High Sensitivity) Assay Kit and qualified using LabChip (LabChip® GX Touch™ PerkinElmer)
测序信息
分子类型: poly(A)+ RNA
库的片段类型: paired
库的链类型: -
测序平台: Illumina
测序仪型号: Illumina HiSeq 2500
链特异性: Unspecific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源 GEN样本编号 GEN数据集编号 系列编号 项目编号 样本编号 样本名称 生物样本编号 样本访问号 实验访问号 释放时间 提交时间 最后更新时间 物种 种族 族裔 年龄 年龄单位 性别 来源名称 组织 细胞类型 细胞亚型 细胞系 疾病 疾病状态 发育阶段 突变/变异 表型 Condition Detail 生长方案 处理方案 提取方案 建库方案 分子类型 库的片段类型 链特异性 库的链类型 加标(Spike-In) 测序方法 测序平台 测序仪型号 细胞数 测序片段数 碱基数 平均测序片段长度_1 平均测序片段长度_2 唯一比对率 多重比对率 覆盖度
文章
Transcriptional Programs Define Intratumoral Heterogeneity of Ewing Sarcoma at Single-Cell Resolution.
Cell reports . 2020-02-01 [PMID: 32049009]