PRJNA533606: Single cell RNA-Seq from bone marrow Ewing patients
概要: We report the application of single-molecule-based sequencing technology for high-throughput profiling of bone marrow Ewing patients
项目整体设计: Study of transcriptomic heterogeneity of Ewing Sarcoma models and comparison with control cell models
| 测序方法: |
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MSCs from bone marrow Ewing patients were isolated by density-gradient centrifugation using Ficoll technique and were cultured in alpha MEM (Gibco), MSC-qualified serum (Gibco), 1% L-glutamine (Gibco) and 1 ng/mL bFGF (Sigma), added ex-tempo. CLB-berlud are cultured in RPMI (Gibco) 10% FBS (Eurobio), 100U/mL. |
| 处理方案: |
No treatment |
| 提取方案: |
Dissociated cells were captured and processed with the C1 Single-Cell Auto Prep System (FluidigmTM) following the manufacturer' protocol |
| 建库方案: |
After harvest from the C1 device, each cDNA library was tagmented using the Nextera XT DNA Sample Preparation Kit (Illumina). Libraries were quantified by Qubit fluorometric assay (Invitrogen) with dsDNA HS (High Sensitivity) Assay Kit and qualified using LabChip (LabChip® GX Touch™ PerkinElmer) |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
paired |
| 库的链类型: |
- |
| 测序平台: |
Illumina |
| 测序仪型号: |
Illumina HiSeq 2500 |
| 链特异性: |
Unspecific |
| 数据来源 |
GEN样本编号 |
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样本编号 |
样本名称 |
生物样本编号 |
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最后更新时间 |
物种 |
种族 |
族裔 |
年龄 |
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性别 |
来源名称 |
组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
疾病状态 |
发育阶段 |
突变/变异 |
表型 |
Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标(Spike-In) |
测序方法 |
测序平台 |
测序仪型号 |
细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Transcriptional Programs Define Intratumoral Heterogeneity of Ewing Sarcoma at Single-Cell Resolution.
Cell reports . 2020-02-01 [PMID:
32049009]