生长方案:	iPSCs were differentiated into definitive endoderm. At day1, medium was changed to RPMI 1640 medium containing 100 ng/mL Activin A and 50 ng/mL bone morphogenetic protein 4, 100 ng/mL Activin A and 0.2% fetal calf serum at day 2, and 100 ng/mL Activin A and 2% FCS at day 3. On Day4-6, cells were cultured in Advanced DMEM/F12 with B27 and N2 containing 500 ng/ml fibroblast growth factor and 3 μM CHIR99021. Cells were maintained at 37 ℃ in 5% CO2 with 95% air and the medium was replaced every day. At day 6, spheroids and attached cells were gently pipetted to be delaminated from dishes. They were centrifuged at 800 rpm for 3 minutes, embedded in a 100% Matrigel drop on the dishes in Advanced DMEM/F12 with B27, N2 and 2 μM retinoic acid, and cultured for 4 days. After RA treatment, the media was switched to Hepatocyte Culture Medium with 10 ng/mL hepatocyte growth factor, 0.1 碌M Dexamethasone and 20 ng/mL Oncostatin M. Cultures for HLO induction were maintained at 37 ℃ in 5% CO2 with 95% air and the medium was replaced every 3 days.
处理方案:	-
提取方案:	17,500 cells were underwent the single cell RNA-seq
建库方案:	Libraries were prepared by the Chromium Single Cell 3' Reagent version 2 kit and Chromium Controller (10X Genomics, CA, USA) as previously described at Zheng, G. X. Y. et al Nature communications 2017.

分子类型:	poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	Forward
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2500
链特异性:	Specific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度