PRJNA540246: The Developmental Heterogeneity of Human Natural Killer Cells Defined by Single-cell Transcriptome
概要: The heterogeneity of human NK cells has not been fully-defined. Using single-cell RNA-sequencing technology, we find more human NK subsets than previously defined by cell surface markers. The transcriptome-based pseudotime analysis support that CD56bright NK cells are precursors of CD56dim NK cells with identification of a transitional stage. Our data significantly expand our understanding of the heterogeneity and development of human NK cells.
项目整体设计: Single-cell RNA-sequencing analysis of NK cells from bone marrow of six healthy donors, blood of two healthy donors and a blood sample from a donor with GATA2T354M mutation
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Cells were washed once with ice-cold PBS containing 10% FBS post-sorting and counted using hemocytometer. After that, the cells were loaded to 10X chromium machine (10X Genomics, Pleasanton, CA) and run through the library preparation procedures following guidance from the Chromium Single Cell 3' Reagent Kits v2. |
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scRNA libraries were prepared for sequencing using the guidance from the 10X Chromium Single Cell 3' Reagent Kits v2 |
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poly(A)+ RNA |
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PAIRED |
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Forward |
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ILLUMINA |
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Illumina NextSeq 500 |
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Specific |
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Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.
Nature communications . 2019-09-02 [PMID:
31477722]
