PRJNA540263: Single cell transcriptomes of developing chicken hind limbs at stages HH25, HH29 and HH31
概要: We sequenced 17’628 cells coming from three key developmental stages of chicken autopod patterning. We identified 23 cell populations with distinct transcriptional profiles, including essential populations like the apical ectodermal ridge. We also inferred gene co-expression modules that coincide with distinct tissue types across developmental time, and used them to track patterning-relevant cell populations of the forming digits.The supplementary file contains processed UMI count matrices, which also include meta data of each cell, e.g. cluster.
项目整体设计: Droplet-based single cell RNA-seq of dissociated developing limbs of chicken at three different stages
| 生长方案: |
- |
| 处理方案: |
- |
| 提取方案: |
Cells were dissociated using trypsin 0.25%. Single cells suspensions were fed into Chromium 10x or DropSeq systems |
| 建库方案: |
DropSeq 10x v2 |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
Forward |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina NextSeq 500 |
| 链特异性: |
Specific |
| 数据来源 |
GEN样本编号 |
GEN数据集编号 |
系列编号 |
项目编号 |
样本编号 |
样本名称 |
生物样本编号 |
样本访问号 |
实验访问号 |
释放时间 |
提交时间 |
最后更新时间 |
物种 |
种族 |
族裔 |
年龄 |
年龄单位 |
性别 |
来源名称 |
组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
疾病状态 |
发育阶段 |
突变/变异 |
表型 |
Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标（Spike-In） |
测序方法 |
测序平台 |
测序仪型号 |
细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
A single-cell transcriptomic atlas of the developing chicken limb.
BMC genomics . 2019-05-22 [PMID:
31117954]
