概要: We report single cell RNA-seq data from patient-derived xenografts that were dissociated, FACS sorted into 96-well plates and profiled by Smart-seq2 and sequencing on an Illumina NextSeq500
项目整体设计: Individual cells from two DIPG cell cultures derived by the Monje lab (DIPG VI and DIPG XIII-FL) were isolated by enzymatic and mechanical digestion followed by FACS sorting. cDNA libraries were generated using Smart-seq2 and Nextera XT with dual unique indexes. Libraries were sequenced on an Illumina NextSeq500, reads aligned by Bowtie and quantified by RSEM and alayses performed using Seurat.
