生长方案:	-
处理方案:	-
提取方案:	Entorhinal cortex tissue from post-mortem Alzheimer's disease and non-disease aged matched individuals was obtained from the Victorian Brain Bank (Ethics Approval for patient tissue banking and consent: University of Melbourne HREC Approval No.: 1545740; approval for transcriptomic analysis using banked tissue Monash University MUHREC 2016-0554; patient demographics in Supplementary Table 1). Nuclei isolation was carried out using the Nuclei Isolation Kit: Nuclei EZ Prep (Sigma, #NUC101) as described in 9. Briefly, tissue samples were homogenized using a glass dounce grinder in 2 ml of ice-cold EZ PREP and incubated on ice for 5 min. Centrifuged nuclei (500 xg, 5 min and 4℃) were washed in ice-cold EZ PREP buffer, and Nuclei Suspension Buffer (NSB; consisting of 1xPBS, 1% (w/v) BSA and 0.2 U/μl RNase inhibitor (Clontech, #2313A). Isolated nuclei were resuspended in NSB to 106 nuclei per 400 μl), filtered through a 40 μm cell strainer and counted with Trypan blue. Nuclei enriched in Nuclei Suspension Buffer were stained with DAPI (1:1000) for nuclei isolation using the Influx cell sorter (BD Biosciences, Franklin Lakes, NJ; 70 μm nozzle, 21-22 psi). Nuclei were defined as DAPI+ singlets. Sorted nuclei were counted twice prior to loading onto the 10X Chromium (10X Genomics).
建库方案:	Library construction was performed using the Chromium Single Cell 3’ Library & Gel Bead Kit v2 (10X Genomics, #PN-120237) with 18 cDNA pre-amplification cycles and sequencing on one high-output lane of the NextSeq 500 (Illumina). Our sequencing saturation was high, ranging from 83.2% to 97.4%.

分子类型:	poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	Forward
测序平台:	ILLUMINA
测序仪型号:	Illumina NextSeq 500
链特异性:	Specific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度