PRJNA579169: RNA-seq analysis of distal gene expression changes during nodulation
概要: Legume plants form symbiotic relationships with diazotrophic bacteria called rhizobia. During such symbiosis, plants provide bacteria with preferred carbon sources such as malate and succinate in return for essential reduced nitrogen. Compatible interactions result in a series of plant root modifications that eventually result in nodule formation. Bacteria living in the nodule cells fix nitrogen via the nitrogenase enzyme complex. Interestingly, as in plant-pathogen interactions, incompatibility in legume-rhizobia associations is also regulated in a genotype-specific manner. For example, the dominant Rj2 gene is presumed to help exclude poor nitrogen-fixing or less-beneficial rhizobia such as B. japonicum USDA122 (U122). The process likely involves recognition of bacterial effectors by host receptor proteins similar to the perception of pathogenic microbes. Our results show that genetic exclusion of incompatible rhizobia in the root requires conserved molecular components of the plant immune response pathway and results in the induction of systemic signaling in the distal tissue. To better understand the mechanism underlying incompatible rhizobia-induced systemic signaling, we compared the transcriptional changes in the foliar tissue of Rj2 plants inoculated with compatible or incompatible rhizobia strains, using RNA-Seq analysis.
项目整体设计: Overall, 1 control (mock), 2 treatments (treated with Incompatible Glycine max L76-1988 with Bradyrhizobium japonicum USDA122 and Glycine max L76-1988 with Sinorhizobium fredii USDA257), each with 3 replicates.
| 生长方案: |
Soybean (Glycine max (L.) Merr.) L76-1988 was grown in the green house with day and night temperatures of 25°C and 20°C, respectively. |
| 处理方案: |
Plants were grown in sterile vermiculite and roots of plants (V1 stage) were inoculated with B. japonicum USDA122 or S. fredii USDA257 (0.5 OD600). |
| 提取方案: |
RNA from leaf tissues was extracted at 24 h post rhizobia inoculation using the TRIzol reagent (Invitrogen, Carlsbad, CA), per manufacturer’s instructions. |
| 建库方案: |
RNA libraries were prepared for sequencing using standard Illumina protocols (TruSeq RNA Library Prep Kit), Paired-end |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
- |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina HiSeq 3000 |
| 链特异性: |
Unspecific |
| 数据来源 |
GEN样本编号 |
GEN数据集编号 |
系列编号 |
项目编号 |
样本编号 |
样本名称 |
生物样本编号 |
样本访问号 |
实验访问号 |
释放时间 |
提交时间 |
最后更新时间 |
物种 |
种族 |
族裔 |
年龄 |
年龄单位 |
性别 |
来源名称 |
组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
疾病状态 |
发育阶段 |
突变/变异 |
表型 |
Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标（Spike-In） |
测序方法 |
测序平台 |
测序仪型号 |
细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Glycerol-3-phosphate mediates rhizobia-induced systemic signaling in soybean.
Nature communications . 2019-11-22 [PMID:
31757957]
