概要: ONAC127 and ONAC129 are NAC transcription factors that involved in abiotic stress response and play key regulatory roles in apoplasmic transportation during rice caryopsis filling. To reveal the transcription regulatory network of ONAC127 and ONAC129 in rice caryopsis, we identified significantly differentially expressed genes by RNA-Seq analysis using the caryopses of the CRISPR-mutants(CR) and overexpression lines(OX) of ONAC127 and ONAC129 under natural heat stress(H) and normal cultivation condition(N).
项目整体设计: Total RNA was extracted from unripe caryopses at 7 DAP by TRIzol Regnant (Invitrogen). For each library, three independent replicated RNA samples were prepared. The RNA samples were then sequenced on the HiSeq 2000 platform
The plants were cultivated in paddy fields under natural long-day conditions (approximately 14 h light/10 h dark) during May to October 2018.
处理方案:
The ambient temperature above 35 ℃ was about 6 h a day and the highest temperature was about 40 ℃ during almost the whole growth period of the natural heat stressed plants, the ambient temperature above 35 ℃ was only 3 days and the mean daytime temperature was about 30 ℃ during the growth period of the normal cultivation condition plants.
提取方案:
Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions.
建库方案:
RNA libraries were prepared for sequencing using standard Illumina protocols.
测序信息
分子类型:
rRNA- RNA
库的片段类型:
PAIRED
库的链类型:
-
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 2000
链特异性:
Unspecific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源
GEN样本编号
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样本名称
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物种
种族
族裔
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来源名称
组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
表型
Condition Detail
生长方案
处理方案
提取方案
建库方案
分子类型
库的片段类型
链特异性
库的链类型
加标（Spike-In）
测序方法
测序平台
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细胞数
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碱基数
平均测序片段长度_1
平均测序片段长度_2
唯一比对率
多重比对率
覆盖度
文章
A heat stress responsive NAC transcription factor heterodimer plays key roles in rice grain filling.
Journal of experimental botany . 2021-04-01 [PMID:
33476364]
