Gene Expression
Nebulas
Gene Expression NebulasSummary: ONAC127 and ONAC129 are NAC transcription factors that involved in abiotic stress response and play key regulatory roles in apoplasmic transportation during rice caryopsis filling. To reveal the transcription regulatory network of ONAC127 and ONAC129 in rice caryopsis, we identified significantly differentially expressed genes by RNA-Seq analysis using the caryopses of the CRISPR-mutants(CR) and overexpression lines(OX) of ONAC127 and ONAC129 under natural heat stress(H) and normal cultivation condition(N).
Overall Design: Total RNA was extracted from unripe caryopses at 7 DAP by TRIzol Regnant (Invitrogen). For each library, three independent replicated RNA samples were prepared. The RNA samples were then sequenced on the HiSeq 2000 platform
| Strategy: |
|
| Species: |
|
| Tissue: |
|
| Healthy Condition: |
|
| Cell Type: |
|
| Cell Line: |
|
| Development Stage: |
|
| Growth Protocol: | The plants were cultivated in paddy fields under natural long-day conditions (approximately 14 h light/10 h dark) during May to October 2018. |
| Treatment Protocol: | The ambient temperature above 35 ℃ was about 6 h a day and the highest temperature was about 40 ℃ during almost the whole growth period of the natural heat stressed plants, the ambient temperature above 35 ℃ was only 3 days and the mean daytime temperature was about 30 ℃ during the growth period of the normal cultivation condition plants. |
| Extract Protocol: | Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. |
| Library Construction Protocol: | RNA libraries were prepared for sequencing using standard Illumina protocols. |
| Molecule Type: | rRNA- RNA |
| Library Source: | |
| Library Layout: | PAIRED |
| Library Strand: | - |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq 2000 |
| Strand-Specific: | Unspecific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Condition Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
|---|