概要: This is an RNA-seq study of the transcriptomic activity of different tissues from adult olive tree cv. Picual on field conditions. This analysis unveiled 53,456 genes with expression in at least one tissue; 32,030 of them were expressed in all organs and 19,575 were found to be potential housekeeping genes. In addition, the specific expression pattern in each part of the plant was studied. The flower was clearly the organ with the highest number of exclusive expressed genes, 3,529, and many of them were involved in reproduction. On the contrary, the stem only showed 229 exclusive expressed genes. The gene ontology (GO) terms analysis of these genes in each organ highlighted some tissue-specific pathways. In general, many of these organ-specific genes are involved in regulatory activities and have nuclear protein localization, with the exception of the leave, where there are also many genes whose coded proteins have plastid localization; to a lesser extent, this was also observed in stems. On the other hand, the pathogen defense and immunity pathways are highly represented in the roots. These data show a complex pattern of gene expression in different organs.
项目整体设计: six plant organs are studied, in two biological replicates each with two technical replicates
Spectrum Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO) used according to the manufacturer’s instructions
建库方案:
A pool of the cDNA libraries was sequenced by paired-end sequencing (101 x 2) with an Illumina HiSeq 2000 sequencer. Two biological replicates per sample were sequenced, and each replicate was loaded twice on two different lanes in the flow cell.
