PRJNA591805: Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum.
概要 : We report transcriptome profiling from three developing stages of anthers in sorghum. Sequencing of nine libraries (three replicates from each stage) yielded >1.12 billion paired-end reads. A total of 31871 known genes expressed in all three replicates of at least one anther stage.
项目整体设计 : Examination of 3 different stages of anther development in Sorghum bicolor variety M35-1
测序方法:
物种:
组织:
高度/长度/重量:
1.6 to 2.0 mm
2.5 to 3.0 mm
Smaller than 1.5 mm
分离条件:
To the supernatant collected in fresh tube
3 volumes of chilled ethanol was added
and tubes were stored at -20 °C overnight for RNA precipitation.
发育阶段:
Meiotic to tetrad
Microspore
Pollen
生长方案:
Soghum bicolor M35-1 plants were raised in an open field
处理方案:
No treatment
提取方案:
Total RNA was isolated using a modified trizol method
建库方案:
Libraries were prepared using TruSeq® Stranded Total RNA kit using manufacturer’s instructions
分子类型:
rRNA- RNA
库的片段类型:
PAIRED
库的链类型:
Forward
测序平台:
ILLUMINA
测序仪型号:
Illumina Genome Analyzer
链特异性:
Specific
数据来源
GEN样本编号
GEN数据集编号
系列编号
项目编号
样本编号
样本名称
生物样本编号
样本访问号
实验访问号
释放时间
提交时间
最后更新时间
物种
种族
族裔
年龄
年龄单位
性别
来源名称
组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
表型
Condition Detail
生长方案
处理方案
提取方案
建库方案
分子类型
库的片段类型
链特异性
库的链类型
加标（Spike-In）
测序方法
测序平台
测序仪型号
细胞数
测序片段数
碱基数
平均测序片段长度_1
平均测序片段长度_2
唯一比对率
多重比对率
覆盖度
Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum. Scientific reports . 2020-01-21 [PMID:
31964983 ]
