Gene Expression
Nebulas
Gene Expression NebulasSummary: We report transcriptome profiling from three developing stages of anthers in sorghum. Sequencing of nine libraries (three replicates from each stage) yielded >1.12 billion paired-end reads. A total of 31871 known genes expressed in all three replicates of at least one anther stage.
Overall Design: Examination of 3 different stages of anther development in Sorghum bicolor variety M35-1
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| Growth Protocol: | Soghum bicolor M35-1 plants were raised in an open field |
| Treatment Protocol: | No treatment |
| Extract Protocol: | Total RNA was isolated using a modified trizol method |
| Library Construction Protocol: | Libraries were prepared using TruSeq® Stranded Total RNA kit using manufacturer’s instructions |
| Molecule Type: | rRNA- RNA |
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| Library Layout: | PAIRED |
| Library Strand: | Forward |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina Genome Analyzer |
| Strand-Specific: | Specific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Condition Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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