PRJNA593861: Single cell transcriptome profiling of the human alcohol-dependent brain samples.
概要: Over 16,000 nuclei were isolated from human postmartum brain frozen prefrontal cortex samples of alcoholic and control individuals. Libraries were prepared with 10X Genomics platform and sequenced using NovaSeq 6000.
项目整体设计: Single nucleus RNA sequencing (snRNA-seq) from prefrontal cortex of 3 alcoholic and 4 control individuals.
| 生长方案: |
- |
| 处理方案: |
Alcoholic and control donors were selected to be as close as possible in terms of age, sex, post-mortem interval (PMI), pH of tissue, and cause of death |
| 提取方案: |
Nuclei were isolated from tissue based on a modified version of Luciano Martelotto's Customer-Developed Protocol provided on the 10XGenomics website. |
| 建库方案: |
Libraries were prepared using the 10X Genomics 3' Single Cell Gene Expression protocol. Paired-end sequencing of the libraries was conducted using a NovaSeq 6000 with an S2 chip (100 cycles). |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
Forward |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina NovaSeq 6000 |
| 链特异性: |
Specific |
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Condition Detail |
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加标（Spike-In） |
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Single cell transcriptome profiling of the human alcohol-dependent brain.
Human molecular genetics . 2020-05-01 [PMID:
32142123]
