Gene Expression Nebulas
A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

Gene Expression Nebulas

A data portal of transcriptome profiles across multiple species
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PRJNA598873

PRJNA598873: Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting

Source: NCBI / GSE143038
Submission Date: Jan 03 2020
Release Date: Jan 04 2020
Update Date: Mar 19 2020

Summary: Using single-cell RNA-sequencing, we comprehensively characterized the transcriptomes of Drosophila olfactory receptor neurons (ORNs) from middle pupal stage antennae. These ORNs are from 3 genotypes: nSyb-GAL4 labels 44 ORN types, 85A10-GAL4 labels 5 ORN types, and AM29-GAL4 labels 2 ORN types.

Overall Design: Single-cell RNA-seq

GEN Datasets:
GEND000068
Strategy:
Species:
Tissue:
Healthy Condition:
Protocol
Growth Protocol: -
Treatment Protocol: -
Extract Protocol: Modified SMART-seq2 (Li et al. 2017)
Library Construction Protocol: -
Sequencing
Molecule Type: poly(A)+ RNA
Library Source:
Library Layout: PAIRED
Library Strand: -
Platform: ILLUMINA
Instrument Model: Illumina NextSeq 550
Strand-Specific: -
Samples
Basic Information:
Sample Characteristic:
Biological Condition:
Experimental Variables:
Protocol:
Sequencing:
Assessing Quality:
Data Resource GEN Sample ID GEN Dataset ID Project ID BioProject ID Sample ID Sample Name BioSample ID Sample Accession Experiment Accession Release Date Submission Date Update Date Species Race Ethnicity Age Age Unit Gender Source Name Tissue Cell Type Cell Subtype Cell Line Disease Disease State Development Stage Mutation Phenotype Condition Detail Growth Protocol Treatment Protocol Extract Protocol Library Construction Protocol Molecule Type Library Layout Strand-Specific Library Strand Spike-In Strategy Platform Instrument Model Cell Number Reads Number Gbases AvgSpotLen1 AvgSpotLen2 Uniq Mapping Rate Multiple Mapping Rate Coverage Rate
Publications
Single-Cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting.
Current biology : CB . 2020-02-13 [PMID: 32059767]