生长方案:	Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~1 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~2 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~3 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~4 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~5 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~6 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~7 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~8 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~9 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~10 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~11 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~12 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~13 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~14 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.; Arabidopsis plants were grown in a growth chamber under the following conditions: long-day photoperiod (16-h light/8-h dark), light intensity 170 μmoles m-2 s-1, day/night temperature 21°C/17 °C. Shortly after bolting (with stem length ~15 cm), the shoot apices were cut and the SAMs were dissected by removing fully open flowers.
处理方案:	-
提取方案:	Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2200 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2201 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2202 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2203 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2204 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2205 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2206 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2207 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2208 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2209 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2210 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2211 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2212 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2213 TapeStation.; Total RNA was isolated from 36 dissected inflorescence meristems using RNeasy Mini Kit (QIAGEN Cat No./ID: 74104), following the manufacturer’s instructions. RNA quality and integrity was assessed on the Agilent 2214 TapeStation.
建库方案:	After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo(dT) beads. For prokaryotic organisms or eukaryotic organisms' long-non-coding libraries, rRNA is removed using the Ribo-Zero kit that leaves the mRNA. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.The qualified libraries are fed into Illumina sequencers after pooling according to its effective concentration and expected data volume.

分子类型:	poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 1000; Illumina HiSeq 1001; Illumina HiSeq 1002; Illumina HiSeq 1003; Illumina HiSeq 1004; Illumina HiSeq 1005; Illumina HiSeq 1006; Illumina HiSeq 1007; Illumina HiSeq 1008; Illumina HiSeq 1009; Illumina HiSeq 1010; Illumina HiSeq 1011; Illumina HiSeq 1012; Illumina HiSeq 1013; Illumina HiSeq 1014
链特异性:	Unspecific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度