概要: In order to better understand the biology of adult schistosomes, we performed single-cell RNAseq on adult male, adult sexually-mature female, and adult virgin female parasites.
项目整体设计: Adult parasites were dissociated then live, single cells were sorted by FACS. Single-cell RNAseq libraries were generated from these cells, then these libraries were sequenced. Further data processing was carried out in Seurat as desribed in the associated manuscript.
Parasites were dissociated and FACS sorted as described in the associated manuscript
建库方案:
single-cell RNAseq libraries were prepared using a 10x genomics Chromium Controller according to the manufacturer's protocol. RNA-Seq (8bp index, 28bp barcode, 58bp insert)
测序信息
分子类型:
poly(A)+ RNA
库的片段类型:
PAIRED
库的链类型:
Forward
测序平台:
ILLUMINA
测序仪型号:
Illumina NextSeq 500
链特异性:
Specific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源
GEN样本编号
GEN数据集编号
系列编号
项目编号
样本编号
样本名称
生物样本编号
样本访问号
实验访问号
释放时间
提交时间
最后更新时间
物种
种族
族裔
年龄
年龄单位
性别
来源名称
组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
表型
Condition Detail
生长方案
处理方案
提取方案
建库方案
分子类型
库的片段类型
链特异性
库的链类型
加标（Spike-In）
测序方法
测序平台
测序仪型号
细胞数
测序片段数
碱基数
平均测序片段长度_1
平均测序片段长度_2
唯一比对率
多重比对率
覆盖度
文章
A single-cell RNA-seq atlas of Schistosoma mansoni identifies a key regulator of blood feeding.
Science (New York, N.Y.) . 2020-09-01 [PMID:
32973030]
