Gene Expression
Nebulas
Gene Expression NebulasSummary: The budding yeast Saccharomyces cerevisiae is a popular host to be used to produce recombinant proteins. Here we studied three yeast strains with different productivity using the RNA-seq data to elucidate the mechanisms for improving protein production.
Overall Design: Three strains were grown under chemostat cultures at two dilution rates, 0.1/h and 0.2/h. Samples used for RNA-seq analysis were collected at the steady state for each condition.
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| Growth Protocol: | Three strains were grown under chemostat cultures at two dilution rates, 0.1/h and 0.2/h. |
| Treatment Protocol: | ACC strain was grown under chemostat cultures at 0.1/h dilution rate, and protein productivity: low.; MH34 strain was grown under chemostat cultures at 0.1/h dilution rate, and protein productivity: medium.; B184 strain was grown under chemostat cultures at 0.1/h dilution rate, and protein productivity: high.; ACC strain was grown under chemostat cultures at 0.2/h dilution rate, and protein productivity: low.; MH34 strain was grown under chemostat cultures at 0.2/h dilution rate, and protein productivity: medium.; B184 strain was grown under chemostat cultures at 0.2/h dilution rate, and protein productivity: high. |
| Extract Protocol: | Refer to QIAGEN RNeasy Mini Handbook |
| Library Construction Protocol: | RNA libraries were prepared for sequencing using standard Illumina protocols |
| Molecule Type: | poly(A)+ RNA |
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| Library Layout: | PAIRED |
| Library Strand: | Forward |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq 4000 |
| Strand-Specific: | Specific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Condition Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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