Gene Expression Nebulas
基于标准化流程分析的转录图谱综合数据库

Gene Expression Nebulas

多物种转录图谱整合数据库

PRJNA613401: RNA-seq data from three Saccharomyces cerevisiae strains with different protein productivity

来源: NCBI / GSE147204
提交时间: Mar 19 2020
释放时间: Oct 26 2020
最后更新时间: Nov 19 2020

概要: The budding yeast Saccharomyces cerevisiae is a popular host to be used to produce recombinant proteins. Here we studied three yeast strains with different productivity using the RNA-seq data to elucidate the mechanisms for improving protein production.

项目整体设计: Three strains were grown under chemostat cultures at two dilution rates, 0.1/h and 0.2/h. Samples used for RNA-seq analysis were collected at the steady state for each condition.

GEN 数据集:
GEND000328
测序方法:
物种:
细胞系:
方案
生长方案: Three strains were grown under chemostat cultures at two dilution rates, 0.1/h and 0.2/h.
处理方案: ACC strain was grown under chemostat cultures at 0.1/h dilution rate, and protein productivity: low.; MH34 strain was grown under chemostat cultures at 0.1/h dilution rate, and protein productivity: medium.; B184 strain was grown under chemostat cultures at 0.1/h dilution rate, and protein productivity: high.; ACC strain was grown under chemostat cultures at 0.2/h dilution rate, and protein productivity: low.; MH34 strain was grown under chemostat cultures at 0.2/h dilution rate, and protein productivity: medium.; B184 strain was grown under chemostat cultures at 0.2/h dilution rate, and protein productivity: high.
提取方案: Refer to QIAGEN RNeasy Mini Handbook
建库方案: RNA libraries were prepared for sequencing using standard Illumina protocols
测序信息
分子类型: poly(A)+ RNA
库的片段类型: PAIRED
库的链类型: Forward
测序平台: ILLUMINA
测序仪型号: Illumina HiSeq 4000
链特异性: Specific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源 GEN样本编号 GEN数据集编号 系列编号 项目编号 样本编号 样本名称 生物样本编号 样本访问号 实验访问号 释放时间 提交时间 最后更新时间 物种 种族 族裔 年龄 年龄单位 性别 来源名称 组织 细胞类型 细胞亚型 细胞系 疾病 疾病状态 发育阶段 突变/变异 表型 Condition Detail 生长方案 处理方案 提取方案 建库方案 分子类型 库的片段类型 链特异性 库的链类型 加标(Spike-In) 测序方法 测序平台 测序仪型号 细胞数 测序片段数 碱基数 平均测序片段长度_1 平均测序片段长度_2 唯一比对率 多重比对率 覆盖度
文章
Different Routes of Protein Folding Contribute to Improved Protein Production in Saccharomyces cerevisiae.
mBio . 2020-11-10 [PMID: 33173005]