PRJNA613401: RNA-seq data from three Saccharomyces cerevisiae strains with different protein productivity
概要: The budding yeast Saccharomyces cerevisiae is a popular host to be used to produce recombinant proteins. Here we studied three yeast strains with different productivity using the RNA-seq data to elucidate the mechanisms for improving protein production.
项目整体设计: Three strains were grown under chemostat cultures at two dilution rates, 0.1/h and 0.2/h. Samples used for RNA-seq analysis were collected at the steady state for each condition.
| 生长方案: |
Three strains were grown under chemostat cultures at two dilution rates, 0.1/h and 0.2/h. |
| 处理方案: |
ACC strain was grown under chemostat cultures at 0.1/h dilution rate, and protein productivity: low.; MH34 strain was grown under chemostat cultures at 0.1/h dilution rate, and protein productivity: medium.; B184 strain was grown under chemostat cultures at 0.1/h dilution rate, and protein productivity: high.; ACC strain was grown under chemostat cultures at 0.2/h dilution rate, and protein productivity: low.; MH34 strain was grown under chemostat cultures at 0.2/h dilution rate, and protein productivity: medium.; B184 strain was grown under chemostat cultures at 0.2/h dilution rate, and protein productivity: high. |
| 提取方案: |
Refer to QIAGEN RNeasy Mini Handbook |
| 建库方案: |
RNA libraries were prepared for sequencing using standard Illumina protocols |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
Forward |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina HiSeq 4000 |
| 链特异性: |
Specific |
| 数据来源 |
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Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标(Spike-In) |
测序方法 |
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碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Different Routes of Protein Folding Contribute to Improved Protein Production in Saccharomyces cerevisiae.
mBio . 2020-11-10 [PMID:
33173005]