概要: We present evidence that (1) viral respiratory infections are potential mechanisms of ACE2 overexpression in patients with asthma and (2) ACE activation regulates multiple cytokine anti-viral responses that could explain a mechanism for cytokine surge and associated tissue damage. These results suggest that the recent finding of severe COVID-19 in asthma patients with recent exacerbations may be attributable to synergistic biomolecular interactions with viral co-infections.
项目整体设计: In a paired design experiments across discovery and validation cohorts of in asthmatic patients the biological replicates treated with RVA and RVC were compared to non-treated ones.
We developed well-differentiated ciliated air-liquid interface (ALI) cultures from nasal tissues biopsied from thirty adults with physician-diagnosed asthma.
处理方案:
-
提取方案:
RNA was extracted from whole-cell lysates, mapped to the UCSC transcript set using Bowtie2 (v2.1.0) and the gene expression level was estimated using standard techniques.
建库方案:
Libraries for RNA-Seq were prepared with Kapa Stranded mRNA. The workflow consisted of mRNA enrichment, cDNA generation, and end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane.
测序信息
分子类型:
rRNA- RNA
库的片段类型:
SINGLE
库的链类型:
Forward
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 3000
链特异性:
Specific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源
GEN样本编号
GEN数据集编号
系列编号
项目编号
样本编号
样本名称
生物样本编号
样本访问号
实验访问号
释放时间
提交时间
最后更新时间
物种
种族
族裔
年龄
年龄单位
性别
来源名称
组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
表型
Condition Detail
生长方案
处理方案
提取方案
建库方案
分子类型
库的片段类型
链特异性
库的链类型
加标（Spike-In）
测序方法
测序平台
测序仪型号
细胞数
测序片段数
碱基数
平均测序片段长度_1
平均测序片段长度_2
唯一比对率
多重比对率
覆盖度
文章
Rhinovirus Infections in Individuals with Asthma Increase ACE2 Expression and Cytokine Pathways Implicated in COVID-19.
American journal of respiratory and critical care medicine . 2020-09-01 [PMID:
32649217]
