生长方案:	-
处理方案:	-; The ovaries samples for scRNA-seq were from two fetal female Macaca fascicularis (embryonic day 84 and 116). For each single cell sequencing experiment, two ovaries were washed twice in 1× PBS, and subjected to a standard digestion procedure through Tumor Dissociation Kit, human (Miltenyi Biotec # 130-095-929). First, the ovaries were cut into small pieces of 2–4 mm, and transferred into the gentleMACS C Tube containing the enzyme mix (4.7 mL DMEM, 200 µL Enzyme H, 100 µL Enzyme R, and 25 µL Enzyme A). Then, the C Tube was attached onto the sleeve of the gentleMACS dissociator. After termination of the program, C Tube was detached from the gentleMACS Dissociator. The sample was incubated for 30 minutes at 37 °C under continuous rotation using the MACSmix Tube Rotator. Next, C Tube was attached onto the sleeve of the gentleMACS dissociator and then perform a short centrifugation step to collect the sample material at the bottom of the tube. Resuspend sample and apply the cell suspension to a MACS SmartStrainer (70 µm) placed on a 50 mL tube and wash cell MACS SmartStrainer (70 µm) with 20 mL DMEM. Finally, centrifuge cell suspension at 300×g for 7 minutes, aspirate supernatant completely and resuspend cells as required for further applications.
提取方案:	Cell instrument to generate a single cell Gel bead in Emulsion(GEM). Single cell RNA libraries were prepared for sequencing according to standard Illumina protocols accompanying the Single Cell 3’ Reagent Kits v3 (10X Genomics)","Cell instrument to generate a single cell Gel bead in Emulsion(GEM). Single cell RNA libraries were prepared for sequencing according to standard Illumina protocols accompanying the Single Cell 3’ Reagent Kits v3 (10X Genomics); RNAs were prepared for sequencing
建库方案:	Then, count matrix was imported into the R package Seurat and quality control was performed to remove outlier cells and genes. Cells with 200-3000 detected genes were retained. Genes were retained in the data if they were expressed in ≥ 3 cells. After applying these quality control criteria, 11742 cells and 19204 genes remained for downstream analyses. Additional normalization was performed in Seurat on the filtered matrix to obtain the normalized count. Highly variable genes across single cells were identified and principal component analysis (PCA) was performed to reduce the dimensionality on the top 18 principal components. Then, cells were clustered at a resolution of 0.6 and visualized in 2-dimension using Uniform Manifold Approximation and Projection (UMAP)." "Then, count matrix was imported into the R package Seurat and quality control was performed to remove outlier cells and genes. Cells with 200-3000 detected genes were retained. Genes were retained in the data if they were expressed in ≥ 3 cells. After applying these quality control criteria, 11742 cells and 19204 genes remained for downstream analyses. Additional normalization was performed in Seurat on the filtered matrix to obtain the normalized count. Highly variable genes across single cells were identified and principal component analysis (PCA) was performed to reduce the dimensionality on the top 18 principal components. Then, cells were clustered at a resolution of 0.6 and visualized in 2-dimension using Uniform Manifold Approximation and Projection (UMAP).; Cell instrument to generate a single cell Gel bead in Emulsion(GEM). Single cell RNA libraries were prepared for sequencing according to standard Illumina protocols accompanying the Single Cell 3’ Reagent Kits v3 (10X Genomics)

分子类型:	Poly(A)+ RNA; polyA(+) RNA
库的片段类型:	PAIRED
库的链类型:	Forward; -
测序平台:	ILLUMINA
测序仪型号:	Illumina NovaSeq 6000
链特异性:	Specific; -

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度