PRJNA633496: Single-cell sequencing reveals heterogeneity effects of arsenic or/and 2,2-dichloroacetamide on zebrafish liver
概要: Arsenic and DBPs has been found to be one of the major risk in many regions of the world. However, current understanding of thier combined toxicities are unclear. Here we used single-cell RNA sequencing to provide the transcriptome heterogeneity of 13563 liver cells obtained from zebrafishes exposed to 100μg/L arsenic, 300μg/L dichloroacetanilide (DCAcAm) and co-exposure for 23 days. Five liver cell populations were identified. We found that the hepatocytes and macrophages were the main target of arsenic and DCAcAm exposure. And the hepatocytes of male and female showed a huge difference, when expsoure to arsenic and DCAcAm).
项目整体设计: Single-cell RNA sequencing were adopted to learn the transcriptional variation in zebrafish liver exposure to arsenic and DCAcAm.
| 生长方案: |
- |
| 处理方案: |
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| 提取方案: |
The isolated liver tissue was digested into cell suspension with dispase. Cells were loaded on a GemCode Single Cell Instrument(10x Genomics, USA) to generate single cell Gel bead in Emulsion (GEMs). |
| 建库方案: |
scRNA-seq libraries were prepared using the GemCode Single-Cell 3’ Gel Bead, Chip and Library Kits (10x Genomics, USA) as per the manufacturer’s protocol. Libraries were sequenced on an Illumina Hiseq PE150. |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
Forward |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina NovaSeq 6000 |
| 链特异性: |
Specific |
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Single-Cell Sequencing Reveals Heterogeneity Effects of Bisphenol A on Zebrafish Embryonic Development.
Environmental science & technology . 2020-07-21 [PMID:
32644799]
