概要: The SF3B complex, a multiprotein component of the U2 snRNP of the spliceosome, mediates branch point selection and facilitates spliceosome assembly and activation. Several splicing modulators bind the SF3B1 and PHF5A subunits of the SF3B complex and globally inhibit splicing. Engineered mutant variants of SF3B1 and PHF5A conferred tolerance to splicing inhibitors with only minor effects on gene expression and AS.
项目整体设计: 7-day-old seedlings of SF3B1, PHF5A and WT are treated for six hours with splicing inhibitor GEX1A
six hours treatment with 0.3 uM Mock; six hours treatment with 0.3 uM GEX1A
提取方案:
Direct-zol RNA MiniPrep Plus (Zymo Research) according to the manufacturer’s instructions
建库方案:
The RNA-seq libraries were constructed using TruSeq mRNA stranded kit following the standard protocol and sequenced on the HiSeq-4000 platform to generate high-quality paired-end reads
