生长方案:	For monolayer-based cardiomyocyte (CMs) differentiation, hESCs were passaged at a density of 3x105cells/well of 6-well plate and grown for 48 hours to 90% confluence in a humidified incubator with 5% CO2 at 37? On day 0, the medium was replaced with RPMI 1640 supplemented with B27 without insulin and 6 M CHIR99021. On day 1, the medium was changed to RPMI 1640 supplemented with B27 without insulin for 48 hr. Day 3, medium was refreshed to RPMI 1640 supplemented with B27 without insulin and 2 M C59 for another 48 hr. On day 5, the medium was changed back to RPMI-B27 without insulin for 48 hr, and then switched to RPMI 1640 plus normal B27 until day 12 with a medium change every 2 days. On day 12, the medium was transiently changed to RPMI 1640 without D-glucose containing ascorbic acid, human albumin and DL-Lactate for two days to allow metabolic purification of CMs. From that day on, fresh RPMI 1640-B27 was changed every two days. On day 21, cells were dissociated with Accutase at 37?followed by resuspending with fresh RPMI 1640-B27 plus Y-27632 and reseeding into 96-well plates. After 24 hr, then switched to RMPI 1640-B27 without Y-27632 for following virus confection tests.; For monolayer-based cardiomyocyte (CMs) differentiation, hESCs were passaged at a density of 3x105cells/well of 6-well plate and grown for 48 hours to 90% confluence in a humidified incubator with 5% CO2 at 37? On day 0, the medium was replaced with RPMI 1640 supplemented with B27 without insulin and 6M CHIR99021. On day 1, the medium was changed to RPMI 1640 supplemented with B27 without insulin for 48 hr. Day 3, medium was refreshed to RPMI 1640 supplemented with B27 without insulin and 2 M C59 for another 48 hr. On day 5, the medium was changed back to RPMI-B27 without insulin for 48 hr, and then switched to RPMI 1640 plus normal B27 until day 12 with a medium change every 2 days. On day 12, the medium was transiently changed to RPMI 1640 without D-glucose containing ascorbic acid, human albumin and DL-Lactate for two days to allow metabolic purification of CMs. From that day on, fresh RPMI 1640-B27 was changed every two days. On day 21, cells were dissociated with Accutase at 37?followed by resuspending with fresh RPMI 1640-B27 plus Y-27632 and reseeding into 96-well plates. After 24 hr, then switched to RMPI 1640-B27 without Y-27632 for following virus confection tests.
处理方案:	hPSC-derived cardiomyocytes were dissociated with Accutase at 37?followed by resuspending with fresh RPMI 1640-B27 plus Y-27632 and reseeding into 96-well plates. After 24 hr, the medium was switched to RMPI 1640-B27 without Y-27632. After 24h recovery, macrophages were added into hPSC-derived cardiomyocytes and co-cultured for another 24 hr before following analysis. Adult cardiomyocytes were also seeded into plates for 48-96 hr and co-cultured with macrophages for 24 hr before following analysis.
提取方案:	null
建库方案:	Libraries were prepared using Illumina TruSeq RNA Sample Preparation (Non-stranded and Poly-A selection).

分子类型:	rRNA- RNA
库的片段类型:	SINGLE
库的链类型:	Forward
测序平台:	ILLUMINA
测序仪型号:	Illumina NextSeq 500; Illumina NovaSeq 6000
链特异性:	Specific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度