概要: We carried out an time-series transcriptome study of susceptible rice cultivar Nipponbare and resistant cultivar Hui1586 in response to M. oryzae isolate Guy11 at 12, 24, 36 and 48 hpi. In total, 48 samples were subjected to RNA-seq, resulting in 6.8-gigabyte 100-bp paired-end reads per sample on average
项目整体设计: mRNA of 14 days rice leaves infected by M. oryzae or water (mock) were subjected for RNA-sequcing and trancriptome profiling analysis.
The rice plants were grown in a light incubator for 14 days with 12 h light and 12 h dark conditions.
处理方案:
Spore concentration was adjusted to 5 × 105 spores/mL with 0.02% Tween-20, H2O with 0.02% Tween-20 was used as mock treatment. The fungal-inoculated rice seedlings were kept in a dark chamber at 25 ◦C with 85% humidity for 24 hours, then the plants were maintained in the growth chamber at 26/24 ◦C in a 14 h light/10 h dark cycle with 85% humidity.
提取方案:
Total RNA quality detection was performed with a Nanodrop for determining the RNA concentration; nondenaturing agarose electrophoresis for determining RNA integrity; and an Agilent 2100 Bioanalyser for determining the total RNA.
建库方案:
RNA libraries were prepared for sequencing using standard Illumina protocols
