生长方案:	nHTBE cells (Lonza Bioscience, CC-2540S) harvested from healthy individuals were expanded using PneumacultEx Plus (Stem Cell Technologies) and seeded on 24 well hanging inserts, 0.4 pore (Corning, USA) and were maintained at ALI for 21+ days in a PneumacultALI growth medium (Stem Cell Technologies) at 37掳C/5% CO2 in a humidified incubator. Cells were used 21+ days post ALI for experiments.
处理方案:	Basal layer of medium was replaced with infection media (DMEM with 2% FBS, 100 U/mL penicillin and streptomycin, 10 mM HEPES, 0.1 mM nonessential amino acids, 4 mM L-glutamate, and 1mM sodium pyruvate). Wells were left untreated, or treated with 100 ng of IFNor 0.1 remdesivir in the basal compartment for 1 hr prior to infection. For infection, SARS-CoV-2 strain 2019-nCoV/USA_WA1/2020 (provided by World Reference Center for Emerging Viruses and Arboviruses at the University of Texas Medical Branch) and icSARS-CoV-2-mNG, were added directly to the apical layer of cells for 1 hr at 37 with rocking every 10 min. The virus was removed from the apical compartment and cells were incubated at 37 until harvest.
提取方案:	Cells were washed and trypsinzed using ReagentPackSubculture Reagents (Lonza Bioscience). Single cell suspensions were washed with PBS + 5% FBS, resuspended in 200 L PBS + 5% FBS, passed through a 70 filter, and placed on ice. Cells were counted using trypan blue exclusion on a Luna II (Logos Biosystems). The appropriate numbers of cells to achieve a targeted cell input of 5,000 cells per condition were used to generate the GEMs (Gel Bead-In Emulsion). The Chromium Next GEM Single Cell 3Gel beads v3.1 kit (10X Genomics, Pleasanton, CA, USA) was used to create GEMs following manufacturer instruction. All samples and reagents were prepared and loaded into the chip and ran in the Chromium Controller for GEM generation and barcoding.
建库方案:	GEMs generated were used for cDNA synthesis and library preparation using the Chromium Single Cell 3 Library Kit v3.1 (10X Genomics) following the manufacturer instruction.; -

分子类型:	Poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina NovaSeq 6000
链特异性:	-

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度