PRJNA663855: Cepharanthine shows antiviral activities by modulating cellular stress responses triggered by a SARS-CoV2 related coronavirus
概要: Antiviral therapies targeting the pandemic coronavirus disease 2019 (COVID 19) are urgently required. We studied an already approved botanical drug cepharanthine (CEP) in a cell culture model of GX_P2V, a severe acute respiratory syndrome coronavirus 2 (SARS CoV 2) related virus. RNA sequencing results showed the virus perturbed the expression of multiple cell stress responses such as endoplasmic reticulum (stress and HSF1 mediated heat shock response related genes and signaling pathways, of which heat shock response related genes and pathways were at the core. CEP was potent to reverse most dysregulated genes and pathways in infected cells mainly by interfering with cellular stress responses including ER stress unfolded protein response and HSF 1 mediated heat shock response . Additionally, single cell transcriptomes from COVID 19 patients (GSE150728 and GSE148829) also confirmed that cellular stress responses and stress induced autophagy play an important role in the development of COVID 19. In summary, this study uncovered the potential mechanisms of the anti-viral activities of CEP, providing evidence for CEP as a promising therapeutic option and modulating cellular stress could be a promising therapy for SARS CoV 2 infection
项目整体设计: Vero E6 cells (1.5×10e7 cells/flask) were plated in T75 culture flasks for 24h. Cells were treated with GX_P2V, CEP, or CEP and virus, and then cultured for another 72h. Cells were harvested and mRNA was isolated by using TRIzon (Invitrogen). The libraries were constructed by using the NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB) standard protocols. The libraries were sequenced on HiSeq 2500 sequencing system (Illumina)
| 测序方法: |
|
| 物种: |
|
| 组织: |
|
| 健康状况: |
|
| 细胞类型: |
|
| 细胞系: |
|
| 发育阶段: |
|
| 生长方案: |
Vero E6 cells (1.5×107 cells/flask) were plated in T75 culture flasks for 24h. |
| 处理方案: |
Cells were treated with GX_P2V (2019-nCoVr at MOI = 0.01), CEP (5 uM), or CEP and virus, and then cultured for another 72h. |
| 提取方案: |
Cells were harvested and mRNA was isolated by using TRIzon (Invitrogen). |
| 建库方案: |
The libraries were constructed by using the NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB) standard protocols. |
| 分子类型: |
Poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
Forward |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina HiSeq 2500 |
| 链特异性: |
Specific |
| 数据来源 |
GEN样本编号 |
GEN数据集编号 |
系列编号 |
项目编号 |
样本编号 |
样本名称 |
生物样本编号 |
样本访问号 |
实验访问号 |
释放时间 |
提交时间 |
最后更新时间 |
物种 |
种族 |
族裔 |
年龄 |
年龄单位 |
性别 |
来源名称 |
组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
疾病状态 |
发育阶段 |
突变/变异 |
表型 |
Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标（Spike-In） |
测序方法 |
测序平台 |
测序仪型号 |
细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Transcriptome analysis of cepharanthine against a SARS-CoV-2-related coronavirus.
Briefings in bioinformatics . 2021-03-01 [PMID:
33423067]
