概要: To characterize the early immune responses against coronavirus infection, we infected rhesus macaques and hACE2 transgenic mice with SARS-CoV-2 and analyzed the transcriptome of infected and non-infected animals. We infected WT mice with IAV as a normal respiratory virus group. During analysis, we found that S100A8 was dramatically upregulated by SARS-CoV-2 and a mouse coronavirus (mouse hepatitis virus, MHV), but not by other tested viruses. A group of non-canonical neutrophils were also activated during SARS-CoV-2 infection.
项目整体设计: WT Rhesus macaques were infected with SARS-CoV-2 intranasally. Lungs were harvested at 0 dpi, 3 dpi, and 5 dpi. RNA extracted from lungs and RNASeq was performed to identify differentially expressed genes; WT C57BL/6J mice and hACE2 transgenic mice were infected with IAV and MHV intranasally. Lungs were harvested at 0 hour, 6 hour, 24 hour, 48 hour, 3 day, 5 day, 7 day post-infection. RNA extracted from lungs and RNASeq was performed to identify differentially expressed genes. Differentially expressed genes in Lungs of WT Rhesus macaques infected with SARS-CoV-2 and WT mice infected with IAV.
All animals were kept and bred in specific pathogen-free conditions.; All animals were kept and bred in specific pathogen-free conditions
处理方案:
Experiments with live SARS-CoV-2 viruses were carried out in the enhanced biosafety level 3 (P3+) facilities. WT Rhesus macaques were inoculated intranasally with SARS-CoV-2 (10^6 TCID50/ml) after anesthesia and lungs were harvested at 0 dpi, 3 dpi and 5 dpi. WT and hACE2 mice were inoculated intranasally with IAV (10^5 TCID50) and MHV (MHV-a59) after anesthesia and lungs were harvested at 0 hour, 6 hour,24, 48hour, 3day, 5day, 7 day post-infection.
提取方案:
Lungs were removed, flash frozen on dry ice, and RNA was harvested using RNeasy Mini Kit (Qiagen NO. 74104).The transcriptome library for sequencing was generated using VAHTSTM mRNA-seq v2 Library Prep Kit for Illumina® (Vazyme Biotech Co.,Ltd, Nanjing, China) following the manufacturer's recommendations
建库方案:
RNA libraries were prepared for sequencing using standard Illumina protocols
测序信息
分子类型:
Poly(A)+ RNA
库的片段类型:
PAIRED
库的链类型:
-; Forward
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 2000
链特异性:
Unspecific; Specific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源
GEN样本编号
GEN数据集编号
系列编号
项目编号
样本编号
样本名称
生物样本编号
样本访问号
实验访问号
释放时间
提交时间
最后更新时间
物种
种族
族裔
年龄
年龄单位
性别
来源名称
组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
表型
Condition Detail
生长方案
处理方案
提取方案
建库方案
分子类型
库的片段类型
链特异性
库的链类型
加标（Spike-In）
测序方法
测序平台
测序仪型号
细胞数
测序片段数
碱基数
平均测序片段长度_1
平均测序片段长度_2
唯一比对率
多重比对率
覆盖度
文章
Induction of alarmin S100A8/A9 mediates activation of aberrant neutrophils in the pathogenesis of COVID-19.
