生长方案:	The pancreatic organs were obtained from the local organ procurement organization under the United Network for Organ Sharing (UNOS). The islets were isolated in the Human Islet Core at University of Pennsylvania following the guidelines of Clinical Islet Transplantation consortium protocol (Ricordi et al., 2016). Briefly, the pancreas was digested following intraductal injection of Collagenase & Neutral Protease in Hanks’ balanced salt solution. Liberated islets were then purified on continuous density gradients (Cellgro/Mediatech) using the COBE 2991 centrifuge and cultured in CIT culture media and kept in a humidified 5% CO2 incubator.
处理方案:	Approximately 1 × 10^6 untreated, control or integrated stress response inhibitor treated (ISRIB) treated, and Sal003 treated primary human pancreatic islet cells were infected with passage-3 SARS-CoV-2 at an MOI of 1 for 24 h in CIT culture media. After 24 hours, infected and mock infected pancreatic islets were dissociated into a single cell suspension using Accutase cell detachment solution (Innovative Cell Technologies) and incubation at 37C for ~20 minutes followed by gentle pipetting to break apart groups of cells.
提取方案:	Target cell inputs of 10,000 cells for each condition were then loaded into a Chromium Controller using Chromium Next GEM (Gel Bead-In Emulsion) single Cell 5’ Library & Gel Bead Kit v1.1 (10x Genomics) according to manufacturer’s instructions. After generation of GEMs, cDNA synthesis and subsequent library preparation of all samples was completed using the Chromium Single Cell 5’ Library Kit v1.1 (10x Genomics) according to manufacturer’s instructions.
建库方案:	The 10X Libraries (Single Cell 5’ R2-only library) were sequenced on the Illumina NovaSeq6000 sequencer with pair-end reads (28 bp for read 1 and 91 bp for read 2).

分子类型:	polyA(+) RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina NovaSeq 6000
链特异性:	-

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度