PRJNA669837: 10X genomics single cell sequencing of sheep testis
概要: A 1.5-year-old healthy male Hu sheep was selected and castrated for testis collection, then single cell suspension was obtained using enzymatic digestion method for single cell sequencing. And the cell types of sheep testis and marker genes of each cell type were identified based on the RNA sequencing.
项目整体设计: mRNA profile of one Hu sheep testis sample was generated by single cell sequencing
| 生长方案: |
- |
| 处理方案: |
- |
| 提取方案: |
The sheep testis tissue was obtained and digested by two-step enzyme digestion, then the single cell suspension was used for sequencing. |
| 建库方案: |
Single testicular cell RNA-seq was performed with a 10X Genomics system using the Illumina HiSeq X Ten platform |
| 分子类型: |
poly(A)+ RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
Forward |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina HiSeq X Ten |
| 链特异性: |
Specific |
| 数据来源 |
GEN样本编号 |
GEN数据集编号 |
系列编号 |
项目编号 |
样本编号 |
样本名称 |
生物样本编号 |
样本访问号 |
实验访问号 |
释放时间 |
提交时间 |
最后更新时间 |
物种 |
种族 |
族裔 |
年龄 |
年龄单位 |
性别 |
来源名称 |
组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
疾病状态 |
发育阶段 |
突变/变异 |
表型 |
Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标（Spike-In） |
测序方法 |
测序平台 |
测序仪型号 |
细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Characterization of sheep spermatogenesis through single-cell RNA sequencing.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology . 2020-11-16 [PMID:
33197070]
