概要: Coronavirus disease 2019 (COVID-19) is the latest respiratory pandemic resulting from zoonotic transmission of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2). Severe symptoms include viral pneumonia secondary to infection and inflammation of the lower respiratory tract, in some cases causing death. We developed primary human lung epithelial 5 infection models to understand responses of proximal and distal lung epithelium to SARS-CoV-2 infection. Differentiated air-liquid interface cultures of proximal airway epithelium and 3D organoid cultures of alveolar epithelium were readily infected by SARS-CoV-2 leading to an epithelial cell-autonomous proinflammatory response. We validated the efficacy of selected candidate COVID-19 drugs confirming that Remdesivir strongly suppressed viral 10 infection/replication. We provide a relevant platform for studying COVID-19 pathobiology and for rapid drug screening against SARS-CoV-2 and future emergent respiratory pathogens.
项目整体设计: Organoid generated from primary lung AT2 cells were either transfected with SARS-CoV2 virus or mock were isolated for mRNAseq
Total RNA was extracted from mock and SARS-CoV-2 infected alveolar organoid cultures lysed in Trizol (Thermo Fisher Scientific) using the chloroform-iso-propanolethanol method.
建库方案:
-; Library construction was performed by the Cedars-Sinai Applied Genomics, Computation and Translational Core using the Lexogen RiboCop rRNA Depletion kit and Swift Biosciences RNA Library Sciences
测序信息
分子类型:
rRNA- RNA
库的片段类型:
SINGLE
库的链类型:
-
测序平台:
ILLUMINA
测序仪型号:
Illumina NovaSeq 6000
链特异性:
Unspecific
样本
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生物条件:
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数据来源
GEN样本编号
GEN数据集编号
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样本名称
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物种
种族
族裔
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性别
来源名称
组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
表型
Condition Detail
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处理方案
提取方案
建库方案
分子类型
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平均测序片段长度_1
平均测序片段长度_2
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多重比对率
覆盖度
文章
SARS-CoV-2 infection of primary human lung epithelium for COVID-19 modeling and drug discovery.
