生长方案:	PBMCs were isolated from blood samples by Ficoll-PaqueTM PLUS density gradient centrifugation and frozen in fetal bovine serum containing 10% (vol/vol) dimethyl sulfoxide. Cells were frozen and not thawed until the day of the experiment when they were used directly for in vitro stimulation.
处理方案:	Cells were stimulated with soluble anti-CD3 and anti-CD28 antibodies at 37 Celsius, 5% CO2 for 24 hours. Unstimulated PBMCs were incubated under identical conditions to reduce any confounding from incubation conditions other than stimulation.
提取方案:	Cells were multiplexed into pools of cells from different patients with the intent of using in silico genetic demultiplexing tools to recover more single cells and reduce batch effect. No samples from the same person were multiplexed into the same pool. Pools were loaded onto the 10x Genomics controller using several lanes per pool, and processed using standard protocols, including cell lysis, reverse transcription, amplification and library generation.
建库方案:	Libraries were constructed using standard protocols from 10x Genomics.

分子类型:	Poly(A)+ RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina NovaSeq 6000
链特异性:	-

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度