概要: Environmental conditions, such as photoperiod and temperature, can affect male fertility in plants. While this feature is heavily exploited in rice to generate male-sterile lines for hybrid breeding, the underlying molecular mechanisms remain largely unknown. In this study, we use a transcriptomics approach to identify key genes and regulatory networks affecting pollen maturation in rice anthers in response to different day lengths.This work provides a new understanding on photoperiodsensitive pollen development in rice, and our gene expression database will provide a new, comprehensive resource to identify new environmentally sensitive genes regulating male fertility for use in crop improvement.
项目整体设计: the mitosis stages I anther were collected at 08:00,12:00,16:00,20:00,00:00, 04:00 in sort-day(12 hours light) and long-day(14 hours dark) condition., each sample have two biological replicates.
Rice variety Oryza sativa L. ssp. japonica 9522 was grown in paddy fields around Shanghai (China) during the normal rice growing season (June–August).
处理方案:
Photoperiod treatment was started after panicle initiation and continued until all samples were collected: LD was the natural daylight condition, with a photoperiod of ~14 h; SD conditions were simulated by covering plants with black cloth for the last 2 h of natural daylight to create a 12 h photoperiod.
提取方案:
RNA was harvested using Trizol reagent (Invitrogen).
建库方案:
RNA library using standard Ion proton RNA-Seq Kit v2.0 protocol
