生长方案:	-
处理方案:	-
提取方案:	For all patients and controls, heparin anticoagulated whole-blood was processed within 4 hours of sampling. PBMCs were separated by density gradient centrifugation using Ficoll-Paque Plus medium (GE Healthcare Life science, Little Chalfont, UK) and washed twice, first with cold PBS and then with cold PBS supplemented with 0.5% sterile endotoxin-free bovine serum albumin (Divbio Science Europe, Breda, the Netherlands). PBMC’s were resuspended in PBS containing 0.5% BSA and 2mm EDTA and the concentration PBMCs was determined using a Coulter Counter. PBMCs were resuspended in IMDM medium containing 20% filter-sterilized fetal calf serum and pen/strep, after which an equal part of the same medium containing 20% DMSO was slowly added while continuously stirring and working on ice. 3-5 million PBMCs were viably stored in 1.8 ml cryogenic vials (Corning #430388), which were steadily brought to -80 degrees Celsius. After 24-72 hours, the cells were transferred to liquid nitrogen storage until further analysis. After thawing, all samples averaged 90% viability as determined by FACS analysis. PBMCs were thawed, stained with viability dye and 500,000 cells were sorted using a FACS Sony Sorter selecting alive, singlet cells. The cells were incubated with FC blocker for 10 minutes, after which each sample was incubated with TotalSeq Hashtag antibody tags to enable multiplexing and subsequent deconvoluting. After Hashtag staining the cells were pooled into four pools of five samples, each sample contributing equally to their respective pool. Cells were counted manually by light microscopy and Neubauer chambers. Each pooled sample was then incubated for 30 minutes with our TotalSeq oligo-conjugated antibody panel (see the Key Resources Table) for later surface protein marker quantification.
建库方案:	Libraries were generated using the Chromium Single Cell 5′ Library & Gel Bead Kit v1.1 (10X genomics, Pleasanton, CA) following the manufacturer’s instructions. In short, pooled cells were loaded aiming the capture of 10,000 cells per pool. Libraries were generated according to standard protocol (Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1 rev E). The amplified mRNA and antibody-derived tags were divided by size and sequenced separately.

分子类型:	polyA(+) RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 4000
链特异性:	-

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度