Gene Expression Nebulas
基于标准化流程分析的转录图谱综合数据库

Gene Expression Nebulas

多物种转录图谱整合数据库

PRJNA694174: Transcriptional analysis of SARS-CoV-2 specific CD4 T cells

来源: NCBI / GSE165373
提交时间: Jan 22 2021
释放时间: May 16 2021
最后更新时间: Aug 16 2021

概要: Transcriptional analysis of SARS-CoV-2 specific CD4 T cells

项目整体设计: The objective of this analysis was to determine if there was a transcriptional difference in the CD4 T cell response elicited by SARS-CoV-2 infection in individuals that quickly resolved their infection compared to those individuals with prolonged SARS-CoV-2-associated symptoms

GEN 数据集:
GEND000454
测序方法:
物种:
组织:
健康状况:
细胞类型:
细胞系:
发育阶段:
方案
生长方案: -
处理方案: Cryopreserved PBMC samples were thawed and resuspended in complete cell culture media at a concentration of 5 × 106 cell/mL and stimulated with 0.5 μg/mL of a SARS-CoV-2 Spike protein peptide pool for 18 h at 37 °C. Spike-reactive CD4+ T cells were identified by expression of the activation markers CD134 and CD69 and isolated by flow cytometric sorting using BD FACSAria II instrument. Cells were sorted directly into 350ul RLT+ buffer supplemented with 1% 2-ME
提取方案: RNA isolated from RLT+ buffer using a RNeasy Micro spin column according to the manufacuter's protocol
建库方案: cDNA was generated using a SMART-Seq HT Kit (TaKaRa, 634455), and the final Illumina-compatible DNA sequencing libraries prepared using an Illumina Nextera XT DNA Library Preparation kit
测序信息
分子类型: polyA(+) RNA
库的片段类型: SINGLE
库的链类型: -
测序平台: ILLUMINA
测序仪型号: Illumina NextSeq 500
链特异性: Unspecific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源 GEN样本编号 GEN数据集编号 系列编号 项目编号 样本编号 样本名称 生物样本编号 样本访问号 实验访问号 释放时间 提交时间 最后更新时间 物种 种族 族裔 年龄 年龄单位 性别 来源名称 组织 细胞类型 细胞亚型 细胞系 疾病 疾病状态 发育阶段 突变/变异 表型 Condition Detail 生长方案 处理方案 提取方案 建库方案 分子类型 库的片段类型 链特异性 库的链类型 加标(Spike-In) 测序方法 测序平台 测序仪型号 细胞数 测序片段数 碱基数 平均测序片段长度_1 平均测序片段长度_2 唯一比对率 多重比对率 覆盖度
文章
Persistent COVID-19 Symptoms Minimally Impact the Development of SARS-CoV-2-Specific T Cell Immunity.
Viruses . 2021-05-15 [PMID: 34063463]