PRJNA694911: Virus-induced senescence is a central pathogenic principle and therapeutic target in COVID-19 disease
概要: SARS-CoV-2 infection accounts for COVID-19 lung disease and other organ manifestations. Increasing evidence points towards an inflammatory cytokine network as the underlying driver of actual organ damage and severity of the clinical course. Here we show that SARS-CoV-2, like a broad spectrum of other viruses, evokes cellular senescence as a primary stress response in infected cells, among them respiratory epithelial cells, which is – indistinguishably from other forms of cellular senescence – characterized by typical morphological and cell-cycle arrest features, and accompanied by the massive secretion of largely pro-inflammatory cytokines, termed senescence-associated secretory phenotype (SASP).
项目整体设计: Examine differentially expressed gene transcripts of senescent cells induced by direct virus infection or conditioned medium from virus-induced senescent cells.
| 生长方案: |
A549 and Wi38 cells were cultivated in DMEM supplemented with 10% FBS and Pen-Strep antibiotics; THP-1 cells were cultivated in RPMI-1640 supplemented with 10% FBS and Pen-Strep antibiotics |
| 处理方案: |
Cells were infected with virus or cultivated in conditioned medium for 5 days (THP-1: 3 days) and cell pellets were collected for RNA extraction. THP1 VIS samples were split into CD86+ and CD86- fractions using surface labelung with a CD86-biotin antibody and anti-biotin Microbeads from Miltenyi, following the manufacturer´s instructions. |
| 提取方案: |
Total RNA was extracted using Qiagen RNeasy Plus Mini Kit |
| 建库方案: |
Libraries were prepared by BGI or DKFZ sequencing core facility according to Illumina's TruSeq RNA kit (RS-122-2001) instructions. |
| 分子类型: |
polyA(+) RNA |
| 库的片段类型: |
SINGLE |
| 库的链类型: |
-; Forward |
| 测序平台: |
BGISEQ; ILLUMINA |
| 测序仪型号: |
DNBSEQ-G400; Illumina HiSeq 4000 |
| 链特异性: |
Unspecific; Specific |
| 数据来源 |
GEN样本编号 |
GEN数据集编号 |
系列编号 |
项目编号 |
样本编号 |
样本名称 |
生物样本编号 |
样本访问号 |
实验访问号 |
释放时间 |
提交时间 |
最后更新时间 |
物种 |
种族 |
族裔 |
年龄 |
年龄单位 |
性别 |
来源名称 |
组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
疾病状态 |
发育阶段 |
突变/变异 |
表型 |
Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标（Spike-In） |
测序方法 |
测序平台 |
测序仪型号 |
细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Virus-induced senescence is a driver and therapeutic target in COVID-19.
Nature . 2021-09-13 [PMID:
34517409]
