概要: The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has gradually become a global public health crisis. Some patients who have recovered from COVID-19 subsequently tested positive again for SARS-CoV-2 after discharge (retesting-positive, RTP). However, the underlying mechanism is unknown. Here, 10 RTP patients, 6 convalescent patients, and 10 healthy controls were enrolled for analysis of the immunological characteristics of their peripheral blood mononuclear cells (PBMCs). We sought to comprehensively characterize the transcriptional changes in the three groups by transcriptome sequencing. Our findings provide insights into the impaired immune function and pathogenesis of RTP occurrence in COVID-19, which may contribute to the development of immunotherapy for RTP patients.
项目整体设计: 10 RTP patients, 6 convalescent patients, and 10 healthy controls were enrolled for analysis of the immunological characteristics of their peripheral blood mononuclear cells (PBMCs)
PBMC were flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
建库方案:
RNA libraries were prepared for sequencing using standard Illumina protocols
测序信息
分子类型:
Poly(A)+ RNA
库的片段类型:
PAIRED
库的链类型:
-
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq X Ten
链特异性:
Unspecific
样本
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数据来源
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组织
细胞类型
细胞亚型
细胞系
疾病
疾病状态
发育阶段
突变/变异
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Condition Detail
生长方案
处理方案
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平均测序片段长度_1
平均测序片段长度_2
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文章
Transcriptomic characteristics and impaired immune function of patients who retest positive for SARS-CoV-2 RNA.
Journal of molecular cell biology . 2021-12-01 [PMID:
34687295]
