PRJNA701942: Next Generation Sequencing Facilitates Quantitative Analysis of Mock/MASCp36 infected mouse lung Transcriptomes
概要: The ongoing SARS-CoV-2 pandemic has brought an urgent need for animal models to study the pathogenicity of the virus. Herein, we generated and characterized a novel mouse-adapted SARS-CoV-2 strain named MASCp36 that causes acute respiratory symptoms and mortality in standard laboratory mice. Particularly, this model exhibits age and gender related skewed distribution of mortality akin to severe COVID-19, and the 50% lethal dose (LD50) of MASCp36 was ~100 PFU in aged, male BALB/c mice. To characterize the transcriptional response to infection of MASCp36 in BALB/c mice (different age and gender), RNA-Seq analysis were performed using lung homogenates collected at 1 and 4 dpi.
项目整体设计: To characterize the transcriptional response to infection of MASCp36 in BALB/c mice (different age and gender), RNA-Seq analysis were performed using lung homogenates collected at 1 and 4 dpi.
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Total RNA from lung were extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) and DNase I (NEB, USA) treated, respectively. |
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Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer’s recommendations |
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total RNA; polyA(+) RNA |
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PAIRED |
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ILLUMINA |
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Illumina NovaSeq 6000 |
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unspecific |
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Immunological Biomarkers of Fatal COVID-19: A Study of 868 Patients.
Frontiers in immunology . 2021-05-03 [PMID:
34012444]
Characterization and structural basis of a lethal mouse-adapted SARS-CoV-2.
Nature communications . 2021-09-27 [PMID:
34580297]
